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Resorufin alpha-D-galactopyranoside
Resorufin alpha-D-galactopyranoside is a sensitive fluorogenic substrate that generates a red fluorescent product (resorufin) upon interaction with alpha-galactosidase. It is used for measuring alpha-galactosidase activities and high throughput screening of alpha-galactosidase inhibitors. Alpha-galactosidase is a glycoside hydrolase enzyme that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. This enzyme is a homodimeric glycoprotein that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. It predominantly hydrolyzes ceramide trihexoside, and it can catalyze the hydrolysis of melibiose into galactose and glucose. A variety of mutations in this gene affect the synthesis, processing, and stability of this enzyme, which causes Fabry's disease, a rare lysosomal storage disorder and sphingolipidosis that results from a failure to catabolize alpha-D-galactosyl glycolipid moieties.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Resorufin alpha-D-galactopyranoside to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 266.432 µL | 1.332 mL | 2.664 mL | 13.322 mL | 26.643 mL |
| 5 mM | 53.286 µL | 266.432 µL | 532.864 µL | 2.664 mL | 5.329 mL |
| 10 mM | 26.643 µL | 133.216 µL | 266.432 µL | 1.332 mL | 2.664 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield |
| Resorufin beta-D-galactopyranoside *CAS 95079-19-9* | 571 | 584 | 650001 | 0.751 |
| Resorufin alpha-D-glucopyranoside *CAS 136565-96-3* | 571 | 584 | 650001 | 0.751 |
References
View all 12 references: Citation Explorer
Preparation of monodisperse and size-controlled poly(ethylene glycol) hydrogel nanoparticles using liposome templates
Authors: An SY, Bui MP, Nam YJ, Han KN, Li CA, Choo J, Lee EK, Katoh S, Kumada Y, Seong GH.
Journal: J Colloid Interface Sci (2009): 98
Authors: An SY, Bui MP, Nam YJ, Han KN, Li CA, Choo J, Lee EK, Katoh S, Kumada Y, Seong GH.
Journal: J Colloid Interface Sci (2009): 98
Synthesis and characterization of a new fluorogenic substrate for alpha-galactosidase
Authors: Shi ZD, Motabar O, Goldin E, Liu K, Southall N, Sidransky E, Austin CP, Griffiths GL, Zheng W.
Journal: Anal Bioanal Chem (2009): 1903
Authors: Shi ZD, Motabar O, Goldin E, Liu K, Southall N, Sidransky E, Austin CP, Griffiths GL, Zheng W.
Journal: Anal Bioanal Chem (2009): 1903
Determination of kinetic parameters, Km and kcat, with a single experiment on a chip
Authors: Jambovane S, Duin EC, Kim SK, Hong JW.
Journal: Anal Chem (2009): 3239
Authors: Jambovane S, Duin EC, Kim SK, Hong JW.
Journal: Anal Chem (2009): 3239
Fast mixing and reaction initiation control of single-enzyme kinetics in confined volumes
Authors: Jung SY, Liu Y, Collier CP.
Journal: Langmuir (2008): 4439
Authors: Jung SY, Liu Y, Collier CP.
Journal: Langmuir (2008): 4439
Photolithographic fabrication of poly(ethylene glycol) microstructures for hydrogel-based microreactors and spatially addressed microarrays
Authors: Baek TJ, Kim NH, Choo J, Lee EK, Seong GH.
Journal: J Microbiol Biotechnol (2007): 1826
Authors: Baek TJ, Kim NH, Choo J, Lee EK, Seong GH.
Journal: J Microbiol Biotechnol (2007): 1826
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