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ReadiUse™ Viral RNA Lysis Buffer
Current demands for RT-PCR testing of SARS-CoV-2 have led to a worldwide shortage of RNA extraction kits. A key missing component required by extraction kit manufacturers and hospitals is the RNA Lysis buffer. This ReadiUse™ Viral RNA Lysis Buffer can be used as an alternative to the Roche MagNA Pure 96 External Lysis Buffer (Code: 06374913001), Biomérieux NucliSENS Lysis Buffer (Code: 280134), and Qiagen Buffer RNAeasy Lysis Buffer (Code: 79216).
Example protocol
PREPARATION OF WORKING SOLUTION
ReadiUse™ Viral RNA Lysis Buffer working solution
Add 0.5 mL of β-Mercaptoethanol to 50 mL of ReadiUse™ Viral RNA Lysis Buffer
Note: Store the RNA Lysis Buffer containing β-Mercaptoethanol at 4 °C.
SAMPLE EXPERIMENTAL PROTOCOL
The following protocol can be used as a guideline and should be optimized according to your experimental needs. This protocol was optimized for use with at least 1.5 X 103 cells to a maximum of 5 X 106 cells per purification.
- Wash the cells once with sterile 1X PBS.
- Add 100 µL of ReadiUse™ Viral RNA Lysis Buffer working solution to the washed cells. Mix by pipetting.
- Purify extracted RNA from the previous step using RNA purification method of choice.
References
View all 50 references: Citation Explorer
Cell Lysis Based on an Oscillating Microbubble Array.
Authors: Liu, Xiufang and Li, Jinyuan and Zhang, Liangyu and Huang, Xiaowei and Farooq, Umar and Pang, Na and Zhou, Wei and Qi, Lin and Xu, Lisheng and Niu, Lili and Meng, Long
Journal: Micromachines (2020)
Authors: Liu, Xiufang and Li, Jinyuan and Zhang, Liangyu and Huang, Xiaowei and Farooq, Umar and Pang, Na and Zhou, Wei and Qi, Lin and Xu, Lisheng and Niu, Lili and Meng, Long
Journal: Micromachines (2020)
PTPN21-CDSlong isoform inhibits the response of acute lymphoblastic leukemia cells to NK-mediated lysis via the KIR/HLA-I axis.
Authors: Wang, Huafang and Zhu, Ni and Ye, Xiaohang and Wang, Limengmeng and Wang, Binsheng and Shan, Wei and Lai, Xiaoyu and Tan, Yamin and Fu, Shan and Xiao, Haowen and Huang, He
Journal: Journal of cellular biochemistry (2020): 3298-3312
Authors: Wang, Huafang and Zhu, Ni and Ye, Xiaohang and Wang, Limengmeng and Wang, Binsheng and Shan, Wei and Lai, Xiaoyu and Tan, Yamin and Fu, Shan and Xiao, Haowen and Huang, He
Journal: Journal of cellular biochemistry (2020): 3298-3312
Lysis of a Lactococcus lactis Dipeptidase Mutant and Rescue by Mutation in the Pleiotropic Regulator CodY.
Authors: Huang, Chenxi and Hernandez-Valdes, Jhonatan A and Kuipers, Oscar P and Kok, Jan
Journal: Applied and environmental microbiology (2020)
Authors: Huang, Chenxi and Hernandez-Valdes, Jhonatan A and Kuipers, Oscar P and Kok, Jan
Journal: Applied and environmental microbiology (2020)
Schistosoma mansoni glyceraldehyde-3-phosphate dehydrogenase enhances formation of the blood-clot lysis protein plasmin.
Authors: Pirovich, David B and Da'dara, Akram A and Skelly, Patrick J
Journal: Biology open (2020)
Authors: Pirovich, David B and Da'dara, Akram A and Skelly, Patrick J
Journal: Biology open (2020)
Transcriptomic responses of the marine cyanobacterium Prochlorococcus to viral lysis products.
Authors: Fang, Xiaoting and Liu, Yaxin and Zhao, Yao and Chen, Yue and Liu, Riyue and Qin, Qi-Long and Li, Gang and Zhang, Yu-Zhong and Chan, Wan and Hess, Wolfgang R and Zeng, Qinglu
Journal: Environmental microbiology (2019): 2015-2028
Authors: Fang, Xiaoting and Liu, Yaxin and Zhao, Yao and Chen, Yue and Liu, Riyue and Qin, Qi-Long and Li, Gang and Zhang, Yu-Zhong and Chan, Wan and Hess, Wolfgang R and Zeng, Qinglu
Journal: Environmental microbiology (2019): 2015-2028
Page updated on November 23, 2024
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