ReadiUse™ TCA Deproteinization Sample Preparation Kit

Important notes
Place kit components on ice before use. 

Protein Precipitation:

1. Prepare a clear solution sample with protein concentration less than 50 mg/mL after homogenization and centrifugation. Keep protein samples on ice. Note: High protein concentration samples might need to be diluted.
   
   
2. Add 10 µL of cold TCA (Component A) into 100 µL of protein sample and vortex briefly to mix well.
   
   
3. Keep the sample on ice for 10 minutes. Centrifuge at 12,000 rpm for 5 minutes. Transfer supernatant (deproteinized sample) to another tube. Note: It is recommended to neutralize samples in TCA and use for relevant assays immediately. However, deproteinized samples in TCA may be stored at -70°C for up to one month.

TCA Neutralization:

1. To neutralize excess TCA, add 10 µL of cold Neutralization Solution (Component B) into the collected supernatant and vortex briefly to mix well.
   
   
2. Keep the samples on ice for 5 minutes. Samples are now deproteinized and neutralized.
   
   
3. (Optional) Read absorbance at 280 nm (OD) to check if the samples are deproteinized.