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ReadiUse™ hydrogen peroxide solution *50 mM calibrated and stabilized solution*
Compared to other commercial hydrogen peroxide solutions, our formulated hydrogen peroxide solution is much more stable. It is calibrated to ensure more reproducible peroxidase-based assays.
Example protocol
AT A GLANCE
Important notes
The following procedure can be adapted for most perioxidase and hydrogen peroxide based assays. In initial experiments, it may be best to try several hydrogen peroxide solutions over the entire suggested range to determine the concentration that yields optimal perioxidase.
PREPARATION OF STOCK SOLUTION
Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles.
1. Peroxidase reaction mixture:
Prepare a Peroxidase reaction mixture with an appropriate hydrogen peroxide substrate concentration and 50 to 500 µM hydrogen peroxide.
SAMPLE EXPERIMENTAL PROTOCOL
- Add equal volume of Peroxidase working solution and peroxidase standards and peroxidase-containing samples.
- Incubate the reaction for 15 to 30 minutes at room temperature, protected from light.
- Monitor the fluorescence or absorbance with appropriate instruments.
References
View all 55 references: Citation Explorer
Development of enzyme-based bar code-style lateral-flow assay for hydrogen peroxide determination
Authors: Fung KK, Chan CP, Renneberg R.
Journal: Anal Chim Acta (2009): 89
Authors: Fung KK, Chan CP, Renneberg R.
Journal: Anal Chim Acta (2009): 89
The accuracy of Amplex Red assay for hydrogen peroxide in the presence of nanoparticles
Authors: Lee CW, Chen YC, Ostafin A.
Journal: J Biomed Nanotechnol (2009): 477
Authors: Lee CW, Chen YC, Ostafin A.
Journal: J Biomed Nanotechnol (2009): 477
Development of a 384-well colorimetric assay to quantify hydrogen peroxide generated by the redox cycling of compounds in the presence of reducing agents
Authors: Johnston PA, Soares KM, Shinde SN, Foster CA, Shun TY, Takyi HK, Wipf P, Lazo JS.
Journal: Assay Drug Dev Technol (2008): 505
Authors: Johnston PA, Soares KM, Shinde SN, Foster CA, Shun TY, Takyi HK, Wipf P, Lazo JS.
Journal: Assay Drug Dev Technol (2008): 505
Hydroxyl radical scavenging assay of phenolics and flavonoids with a modified cupric reducing antioxidant capacity (CUPRAC) method using catalase for hydrogen peroxide degradation
Authors: Ozyurek M, Bektasoglu B, Guclu K, Apak R.
Journal: Anal Chim Acta (2008): 196
Authors: Ozyurek M, Bektasoglu B, Guclu K, Apak R.
Journal: Anal Chim Acta (2008): 196
Homogeneous, unmodified gold nanoparticle-based colorimetric assay of hydrogen peroxide
Authors: Wu ZS, Zhang SB, Guo MM, Chen CR, Shen GL, Yu RQ.
Journal: Anal Chim Acta (2007): 122
Authors: Wu ZS, Zhang SB, Guo MM, Chen CR, Shen GL, Yu RQ.
Journal: Anal Chim Acta (2007): 122
Page updated on November 21, 2024
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