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ReadiUse™ Cell Detaching Buffer
ReadiUse™ cell detaching buffer is a cell detachment solution that does not contain mammalian or bacterial-derived products. It works similar to trypsin with much less toxic effect. It performs exceptionally well in detaching primary and stem cells and maintains high cell viability. ReadiUse™ cell detaching buffer is very useful for routine cell passage, analysis of cell surface markers and receptors, cell proliferation, apoptosis and flow cytometry.
Example protocol
SAMPLE EXPERIMENTAL PROTOCOL
Note:
The ReadiUse™ Cell Detaching Buffer is supplied as a sterile, ready-to use, frozen liquid. After thawing, It may be stored for up to 2 months at 4 °C.
The following protocol can be used as a guideline and can be modified as per user's need.
Sample protocol
Thaw the ReadiUse™ cell detaching buffer at room temperature.
Aspirate all of the media from the cell culture dish or flask. Note: It is not necessary to wash PBS.
Add the ReadiUse™ cell detaching buffer to the culture dish or flask to cover the cells, typically using ~10 mL for each 75 cm2 of surface area.
Return culture to 37 °C incubator and allow cells to detach for 5–10 minutes.
Count cells and passage as usual, no additional washes or enzyme inhibitors are required.
Citations
View all 30 citations: Citation Explorer
A Simple Cell-based Immunofluorescence Assay to Detect Autoantibody Against the N-Methyl-D-Aspartate (NMDA) Receptor in Blood
Authors: Chen, C. H., Chang, Y. S.
Journal: J Vis Exp (2018): se name="60010.enl" path="C:\Website\Referenc
Authors: Chen, C. H., Chang, Y. S.
Journal: J Vis Exp (2018): se name="60010.enl" path="C:\Website\Referenc
The virtual cell based assay: Current status and future perspectives
Authors: Graepel, R., Lamon, L., Asturiol, D., Berggren, E., Joossens, E., Paini, A., Prieto, P., Whelan, M., Worth, A.
Journal: Toxicol In Vitro (2017): 258-267
Authors: Graepel, R., Lamon, L., Asturiol, D., Berggren, E., Joossens, E., Paini, A., Prieto, P., Whelan, M., Worth, A.
Journal: Toxicol In Vitro (2017): 258-267
Evaluation of anti-Zika virus activities of broad-spectrum antivirals and NIH clinical collection compounds using a cell-based, high-throughput screen assay
Authors: Adcock, R. S., Chu, Y. K., Golden, J. E., Chung, D. H.
Journal: Antiviral Res (2017): 47-56
Authors: Adcock, R. S., Chu, Y. K., Golden, J. E., Chung, D. H.
Journal: Antiviral Res (2017): 47-56
A cell-based high-throughput screening assay system for inhibitor compounds of antigen presentation by HLA class II molecule
Authors: Watanabe, N., Suzuki, Y., Yonezu, T., Nakagawa, Y., Shiina, T., Hirayama, N., Inokuchi, S., Inoue, S.
Journal: Sci Rep (2017): 6798
Authors: Watanabe, N., Suzuki, Y., Yonezu, T., Nakagawa, Y., Shiina, T., Hirayama, N., Inokuchi, S., Inoue, S.
Journal: Sci Rep (2017): 6798
Fluorescent Parkin Cell-Based Assay Development for the Screening of Drugs against Parkinson Disease
Authors: Villace, P., Mella, R. M., Roura-Ferrer, M., Valcarcel, M., Salado, C., Castilla, A., Kortazar, D.
Journal: SLAS Discov (2017): 67-76
Authors: Villace, P., Mella, R. M., Roura-Ferrer, M., Valcarcel, M., Salado, C., Castilla, A., Kortazar, D.
Journal: SLAS Discov (2017): 67-76
Page updated on November 20, 2024
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