ReadiPrep™ PCR Purification Kit

Product key features

High recovery: Achieve high-recovery of purified DNA from PCR amplification products.
All-in-one kit: Pre-packed spin columns and optimized buffers included.
Multipurpose: Compatible with PCR products, DNA restriction enzyme digests, and other enzymatic reactions.
Rapid protocol: Complete DNA purification in less than 10 minutes.

Product description

The ReadiPrep™ PCR Purification Kit is a fast and reliable DNA cleanup system designed for the purification of amplified DNA from PCR reactions. Using a silica membrane-based spin column, the kit efficiently removes primers, unused dNTPs, salts, enzymes, and other contaminants to deliver high-purity DNA ready for downstream applications.

This PCR cleanup kit is optimized for a wide range of molecular biology workflows including DNA sequencing, cloning, ligation, and quantitative PCR (qPCR). Its user-friendly protocol minimizes hands-on time and ensures consistent, reproducible results—ideal for both routine and high-throughput labs. The ReadiPrep™ PCR Purification Kit offers the speed, simplicity, and performance required for efficient and high-quality DNA recovery.

Example protocol

AT A GLANCE

Add 5 volumes of ReadiPrep™ Binding Buffer into PCR sample.
Apply the mix to ReadiPrep™ Spin Column and centrifuge the column.
Wash the column with ReadiPrep™ Wash Buffer working solution.
Elute the sample from the column with ReadiPrep™ Elution Buffer.

PREPARATION OF WORKING SOLUTION

Prepare ReadiPrep™ Wash Buffer working solution by adding 200 μL of ReadiPrep™ Wash Buffer (Component C) into 800 μL of Ethanol (96-100%, not provided).

Note: 1 mL of working solution is enough for 1 prep.

SAMPLE EXPERIMENTAL PROTOCOL

Add 5 volumes of ReadiPrep™ Binding Buffer (Component A) to 1 volume of the PCR sample, and then mix well. E.g. Add 500 μL of ReadiPrep™ Binding Buffer to 100 μL of PCR sample.
Note: For samples lower than 100 μL volume, add molecular grade water to make up the volume to 100 μL.
Apply the sample to ReadiPrep™ Spin Column (Component B) and centrifuge the column at 13,000 rpm for 60 seconds.
Note: For better binding, samples in columns can be incubated for 1 minute before centrifugation.
Discard the flow-through and place the column back into the collection tube.
Add 600 μL of ReadiPrep™ Wash Buffer working solution and centrifuge the column at 13,000 rpm for 60 seconds.
Note: For additional salt removal, wash buffer in columns can be incubated for 1 minute before performing centrifuge. Note: To prevent salt carryover for sensitive procedures, additional wash steps can be performed with 350 μL of ReadiPrep™ Wash Buffer working solution and repeat step-4.
Remove the wash buffer and perform an additional centrifuge at 13,000 rpm for 2 minutes to dry the columns completely.
Add 30 μL of ReadiPrep™ Elution Buffer (Component D) to the center of the column and centrifuge the column at 13,000 rpm for 60 seconds. To increase yield, perform another elution step with 30 μL of ReadiPrep™ Elution Buffer and centrifuge. Note: For better yield, elution buffer in columns can be incubated for 1 minute before performing centrifuge.

References

View all 25 references: Citation Explorer

Protocol to retrieve unknown flanking DNA sequences using semi-site-specific PCR-based genome walking.
Authors: Chen, Hong and Wei, Cheng and Lin, Zhiyu and Pei, Jinfen and Pan, Hao and Li, Haixing
Journal: STAR protocols (2024): 102864

Comparative analysis of DNA extraction and PCR product purification methods for cervicovaginal microbiome analysis using cpn60 microbial profiling.
Authors: Shvartsman, Elinor and Richmond, Meika E I and Schellenberg, John J and Lamont, Alana and Perciani, Catia and Russell, Justen N H and Poliquin, Vanessa and Burgener, Adam and Jaoko, Walter and Sandstrom, Paul and MacDonald, Kelly S
Journal: PloS one (2022): e0262355

Disproportionate Distribution of HBV Genotypes A and D and the Recombinant Genotype D/E in the High and Low HBV Endemic Regions of Uganda: A Wake-Up Call for Regional Specific HBV Management.
Authors: Kafeero, Hussein Mukasa and Ndagire, Dorothy and Ocama, Ponsiano and Kato, Charles Drago and Wampande, Eddie and Kajumbula, Henry and Kateete, David and Walusansa, Abdul and Kudamba, Ali and Edgar, Kigozi and Katabazi, Fred Ashaba and Namaganda, Maria Magdalene and Ssenku, Jamilu E and Sendagire, Hakim
Journal: International journal of hepatology (2022): 3688547

Evaluation of methods for plant genomic DNA sequence analysis without DNA and PCR product purification.
Authors: Matsuo, Kouki
Journal: Plant science : an international journal of experimental plant biology (2021): 111023

Human Papillomavirus Investigation in Head and Neck Squamous Cell Carcinoma: Initial Report from the Low Risk HPV Types Associations.
Authors: Karbalaie Niya, Mohammad Hadi and Safarnezhad Tameshkel, Fahimeh and Panahi, Mahshid and Bokharaei Salim, Farah and Monavari, Seyed Hamid Reza and Keyvani, Hossein
Journal: Asian Pacific journal of cancer prevention : APJCP (2017): 2573-2579

Page updated on April 15, 2025

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