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ReadiPrep™ Mycoplasma Inhibitor Solution *1000X*

ReadiPrep™ Mycoplasma Inhibitor Solution is a sterile, concentrated (1000x) antibiotic/antimycotic solution. It is cell culture tested and ready to use with any standard cell culture media. ReadiPrep™ Mycoplasma Inhibitor Solution requires no special protective equipment needed other than for standard cell culture. It is compatible with most commonly used cell lines.
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Unit size
5x1 mL
25 mL
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Additional ordering information
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Emailsales@aatbio.com
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Physical properties
SolventWater
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C); Minimize light exposure
Example protocol

SAMPLE EXPERIMENTAL PROTOCOL

This product is safe to use at the recommended concentration. No special protective equipment is required beyond standard cell culture practices. For more information, please refer to the safety data sheet.

  1. Add 1.0 mL of ReadiPrep™ Mycoplasma Inhibitor Solution 1000x to 1 liter of cell culture media. Use the prepared working media within one month.

    Note: For certain cell lines, it may be necessary to test different dilutions (e.g., 0.5x, 1x, 2x, and 4x). 

    Note: Use MycoLytics without any additional antibiotics in the media. Maintain appropriate cell densities, subculture the cells four times, and then retest.

References
View all 56 references: Citation Explorer
Biochemical and functional characterization of heat-inactivated coelomic fluid from earthworms as a potential alternative for fetal bovine serum in animal cell culture.
Authors: Rossan Mathews, Melinda Grace and Subramaniam, Ravichandran and Venkatachalam, Saravanakumar and Selvan Christyraj, Johnson Retnaraj Samuel and Yesudhason, Beryl Vedha and Kalimuthu, Kalishwaralal and Mohan, Manikandan and Selvan Christyraj, Jackson Durairaj
Journal: Scientific reports (2024): 5606
In vitro modelling of bacterial pneumonia: a comparative analysis of widely applied complex cell culture models.
Authors: Mahieu, Laure and Van Moll, Laurence and De Vooght, Linda and Delputte, Peter and Cos, Paul
Journal: FEMS microbiology reviews (2024)
Comparative genomic analysis of Mycoplasma related to cell culture for infB gene-based loop-mediated isothermal amplification.
Authors: Luna-Callejas, Benjamín and Oropeza-Ramos, Laura and Ramón-Gallegos, Eva
Journal: World journal of microbiology & biotechnology (2023): 355
Cell Culture Techniques and Practices to Avoid Contamination by Fungi and Bacteria in the Research Cell Culture Laboratory.
Authors: Tanasescu, Ana-Maria
Journal: Journal of visualized experiments : JoVE (2023)
A Beginner's Guide to Cell Culture: Practical Advice for Preventing Needless Problems.
Authors: Weiskirchen, Sabine and Schröder, Sarah K and Buhl, Eva Miriam and Weiskirchen, Ralf
Journal: Cells (2023)