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ReadiLink™ Rapid mFluor™ Violet 610 Antibody Labeling Kit
Microscale Optimized for Labeling 50 µg Antibody Per Reaction
ReadiLink™ Rapid mFluor™ Violet 610 Antibody Labeling Kits provide a quick and convenient method to label antibodies, proteins (>10 kDa), or other amine-containing biomolecules. Leveraging a unique conjugation chemistry, ReadiLink™ Antibody Labeling Kits enables researchers to label microscale volumes of antibodies in two easy steps, with no purification needed and 100% recovery of the labeled product. Each kit includes sufficient mFluor™ Violet 610 dye to facilitate two distinct labeling reactions of 50 µg antibody samples. The resulting conjugates are ideal for a wide range of applications, including flow cytometry, fluorescent microscopy techniques, immunocytochemistry (ICC), immunohistochemistry (IHC), ELISA, and indirect FISH. Compared to Alexa Fluor dyes and conventional dye counterparts, mFluor™ dyes are brighter, more photostable, and resistance to changes in pH between pH 4 to 10. The mFluor™ Violet 610 dyes are optimally excited by violet lasers and emit red fluorescence ~610 nm. These properties make them well-suited for spectral fluorescence flow cytometry, providing researchers with a tool for detailed biological analysis with unparalleled precision and sensitivity. With ReadiLink™ Rapid Antibody Labeling kits, researchers can directly label primary antibodies, eliminating the need for secondary antibodies and enhancing panel-building flexibility.

 

readilinkworkflow

 

Figure 1. Overview of the ReadiLink™ Rapid Antibody Labeling protocol. In just two simple steps, and with no purification necessary, covalently label microgram amounts of antibodies in under an hour.

Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to generate four distinct emission profiles, then, when combined, yielded the overall spectral signature. Bottom) Flow cytometry analysis of whole blood cells stained with CD4-mFluor™ Violet 610 conjugate. Conjugates were prepared using the ReadiLink™ Rapid mFluor™ Violet 610 Antibody Labeling Kit. The fluorescence signal was monitored using a Cytek® Aurora spectral flow cytometer in the mFluor™ Violet 610 specific V10-A channel.
Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to generate four distinct emission profiles, then, when combined, yielded the overall spectral signature. Bottom) Flow cytometry analysis of whole blood cells stained with CD4-mFluor™ Violet 610 conjugate. Conjugates were prepared using the ReadiLink™ Rapid mFluor™ Violet 610 Antibody Labeling Kit. The fluorescence signal was monitored using a Cytek® Aurora spectral flow cytometer in the mFluor™ Violet 610 specific V10-A channel.
CatalogSize
Price
Quantity
11162 Labelings
Price
 
Spectral properties

Absorbance (nm)594
Correction factor (260 nm)0.532
Correction factor (280 nm)0.66
Extinction coefficient (cm -1 M -1)
90000
1
Excitation (nm)421
Emission (nm)612
Quantum yield
0.3
1
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
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Page updated on October 15, 2025