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ReadiLink™ Protein Conjugation Stop Buffer
ReadiLink™ Protein Conjugation Stop Buffer is used to stop the protein conjugation reactions that use succinimidyl ester, sulfonyl chloride or isothiocyanate. It can be used directly without dilution.
Example protocol
AT A GLANCE
Important Warm up ReadiLink™ protein conjugation stop buffer at room temperature before use.
SAMPLE EXPERIMENTAL PROTOCOL
- Prepare your protein (antibody) conjugation reaction as needed.
- Stop Conjugation reaction by adding ReadiLink™ protein conjugation stop buffer at 1:50 ratio (such as for a 100 µL conjugation reaction, add 2 µL ReadiLink™ protein conjugation stop buffer).
- Incubate at room temperature for 10 minutes.
- The labeled protein (antibody) is now ready to use.
Note It is not necessary to purify conjugated proteins (antibodies) with a column after the conjugation reaction is stopped with ReadiLink™ protein conjugation stop buffer if the conjugation is used within 1 month.
Note For long term storage, it is recommended purifying the conjugated proteins (antibodies) with desalting column. And the protein conjugate should be stored at > 0.5 mg/mL in the presence of a carrier protein (e.g., 0.1% bovine serum albumin). For longer storage, the protein conjugates could be lyophilized or divided into single-used aliquots and stored at ≤ –20°C.
Page updated on November 21, 2024
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