ReadiLink™ iFluor® 647 Oligo and ssDNA Labeling Kit

Name: ReadiLink™ iFluor® 647 Oligo and ssDNA Labeling Kit
Brand: AAT Bioquest
SKU: 17484
Availability: InStock

ReadiLink™ iFluor® 647 Oligo and ssDNA Labelling Kit enables simple and uniform tagging of single-stranded DNA or oligos with iFluor® 647, our bright, photostable and green-fluorescent fluorophore. The labelling kit uses our proprietary TAQuest™ terminal deoxynucleotidyl transferase (TdT) to catalyze non-template directed nucleotide incorporation onto the 3’- end of single-stranded DNAs or oligos. The kit is optimized for efficient labelling and contains all the essential reagents required for efficient labelling of ssDNA or oligos. The resulting iFluor® 647-labelled DNA probes are ideally suited for biological applications, e.g., electrophoretic mobility shift assays (EMSA), Northern and Southern blots, colony or in situ hybridizations.

Example protocol

AT A GLANCE

Protocol summary

Prepare oligo or ssDNA samples
Add reagents to tube
Mix and centrifuge briefly
Incubate at 37 °C for 60 minutes
Place on ice for 5 minutes
Purify the labeled DNA

Note: Thaw all the kit components on ice before starting the experiment. Briefly centrifuge all the reagents to the bottom before starting the labeling process.

SAMPLE EXPERIMENTAL PROTOCOL

The following protocol can be used as a guideline.

To a clean (Nuclease-free) 0.5 mL microcentrifuge tube or 0.2 mL PCR tube, prepare a reaction mix by adding the reagents in the order indicated in Table 1.
Carefully mix the reagents by a brief vortex, followed by a brief centrifuge.
Incubate the reaction at 37 °C for 60 minutes.
After incubation, place the reaction on ice for 5 minutes.
Purify the labeled DNA.

Table 1.Reagents composition per tube for each reaction

Components	Amount
Oligo or ssDNA sample	1 µg DNA diluted in Nuclease-free water to a final volume of 5 µL
TdT Reaction Buffer	40 µL
iFluor™ 647-dUTP	1-2 µL
CoCl₂	5 µL
TdT enzyme	0.5 µL
Total Volume	52 µL (Approx.)

Note: The amount of iFluor™ 647-dUTP can be optimized to achieve the best labeling conditions.

Spectrum

Open in Advanced Spectrum Viewer

Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
ReadiLink™ iFluor® 488 Oligo and ssDNA Labeling Kit	491	516	75000¹	0.9¹	0.21	0.11
ReadiLink™ iFluor® 555 Oligo and ssDNA Labeling Kit	557	570	100000¹	0.64¹	0.23	0.14

References

View all 13 references: Citation Explorer

A Ratiometric Fluorescent Conjugated Oligomer for Amyloid β Recognition, Aggregation Inhibition, and Detoxification.
Authors: Zhang, Ziqi and Yuan, Qiong and Li, Meiqi and Bao, Benkai and Tang, Yanli
Journal: Small (Weinheim an der Bergstrasse, Germany) (2021): e2104581

Chorus2: design of genome-scale oligonucleotide-based probes for fluorescence in situ hybridization.
Authors: Zhang, Tao and Liu, Guanqing and Zhao, Hainan and Braz, Guilherme T and Jiang, Jiming
Journal: Plant biotechnology journal (2021): 1967-1978

General Method for Determining Redox Potentials without Electrolyte.
Authors: Bird, Matthew J and Pearson, Matthew A and Asaoka, Sadayuki and Miller, John R
Journal: The journal of physical chemistry. A (2020): 5487-5495

Breaking Parallel Orientation of Rods via a Dendritic Architecture toward Diverse Supramolecular Structures.
Authors: Zhang, Ruimeng and Feng, Xueyan and Zhang, Rui and Shan, Wenpeng and Su, Zebin and Mao, Jialin and Wesdemiotis, Chrys and Huang, Jiahao and Yan, Xiao-Yun and Liu, Tong and Li, Tao and Huang, Mingjun and Lin, Zhiwei and Shi, An-Chang and Cheng, Stephen Z D
Journal: Angewandte Chemie (International ed. in English) (2019): 11879-11885

Manufacture of Clinical-Grade Human Clonal Mesenchymal Stem Cell Products from Single Colony Forming Unit-Derived Colonies Based on the Subfractionation Culturing Method.
Authors: Yi, TacGhee and Kim, Si-na and Lee, Hyun-Joo and Kim, Junghee and Cho, Yun-Kyoung and Shin, Dong-Hee and Tak, Sun-Ji and Moon, Sun-Hwa and Kang, Ji-Eun and Ji, In-Mi and Lim, Huyn-Ja and Lee, Dong-Soon and Jeon, Myung-Shin and Song, Sun U
Journal: Tissue engineering. Part C, Methods (2015): 1251-62

Page updated on December 17, 2024

Ordering information

Price
Unit size	10 Reactions 20 Reactions
Catalog Number	1748417485
Quantity

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
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Spectral properties

Correction Factor (260 nm)	0.03
Correction Factor (280 nm)	0.03
Correction Factor (656 nm)	0.0793
Extinction coefficient (cm ^-1 M ^-1)	250000¹
Excitation (nm)	656
Emission (nm)	670
Quantum yield	0.25¹