ReadiLink™ Cy3 Oligo and ssDNA Labeling Kit

ReadiLink™ Cy3 Oligo and ssDNA Labelling Kit enables simple and uniform tagging of single-stranded DNA or oligos with Cy3 fluorophore. The labelling kit uses our proprietary TAQuest™ terminal deoxynucleotidyl transferase (TdT) to catalyze non-template directed nucleotide incorporation onto the 3’- end of single-stranded DNAs or oligos. The kit is optimized for efficient labelling and contains all the essential reagents required for efficient labelling of ssDNA or oligos. The resulting Cy3-labelled DNA probes are ideally suited for biological applications, e.g., electrophoretic mobility shift assays (EMSA), Northern and Southern blots, colony or in situ hybridizations.

Example protocol

AT A GLANCE

Protocol summary

Prepare oligo or ssDNA samples
Add reagents to tube
Mix and centrifuge briefly
Incubate at 37 °C for 60 minutes
Place on ice for 5 minutes
Purify the labeled DNA

Note: Thaw all the kit components on ice before starting the experiment. Briefly centrifuge all the reagents to the bottom before starting the labeling process.

SAMPLE EXPERIMENTAL PROTOCOL

The following protocol can be used as a guideline.

To a clean (Nuclease-free) 0.5 mL microcentrifuge tube or 0.2 mL PCR tube, prepare a reaction mix by adding the reagents in the order indicated in Table 1.
Carefully mix the reagents by a brief vortex, followed by a brief centrifuge.
Incubate the reaction at 37 °C for 60 minutes.
After incubation, place the reaction on ice for 5 minutes.
Purify the labeled DNA.

Table 1.Reagents composition per tube for each reaction

Components	Amount
Oligo or ssDNA sample	1 µg DNA diluted in Nuclease-free water to a final volume of 5 µL
TdT Reaction Buffer	40 µL
Cy3-dUTP	1-2 µL
CoCl₂	5 µL
TdT enzyme	0.5 µL
Total Volume	52 µL (Approx.)

Note: The amount of Cy3-dUTP can be optimized to achieve the best labeling conditions.

Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)	Correction Factor (482 nm)	Correction Factor (565 nm)
ReadiLink™ Cy5 Oligo and ssDNA Labeling Kit	651	670	250000¹	0.27¹, 0.4²	0.02	0.03	0.009	0.09

References

View all 50 references: Citation Explorer

Frequency-Upconverted Stimulated Emission by Up to Six-Photon Excitation from Highly Extended Spiro-Fused Ladder-Type Oligo(p-phenylene)s.
Authors: Jiang, Yi and Li, King Fai and Gao, Kun and Lin, He and Tam, Hoi Lam and Liu, Yuan-Yuan and Shu, Yu and Wong, Ka-Leung and Lai, Wen-Yong and Cheah, Kok Wai and Huang, Wei
Journal: Angewandte Chemie (International ed. in English) (2021): 10007-10015

Oligo-FISH barcode in beans: a new chromosome identification system.
Authors: de Oliveira Bustamante, Fernanda and do Nascimento, Thiago Henrique and Montenegro, Claudio and Dias, Sibelle and do Vale Martins, Lívia and Braz, Guilherme Tomaz and Benko-Iseppon, Ana Maria and Jiang, Jiming and Pedrosa-Harand, Andrea and Brasileiro-Vidal, Ana Christina
Journal: TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik (2021): 3675-3686

Distribution of FISH oligo-5S rDNA and oligo-(AGGGTTT)3 in Hibiscus mutabilis L.
Authors: Luo, Xiaomei and He, Zhoujian
Journal: Genome (2021): 655-664

Identification of 5S and 45S rDNA sites in Chrysanthemum species by using oligonucleotide fluorescence in situ hybridization (Oligo-FISH).
Authors: He, Jun and Lin, Sisi and Yu, Zhongyu and Song, Aiping and Guan, Zhiyong and Fang, Weimin and Chen, Sumei and Zhang, Fei and Jiang, Jiafu and Chen, Fadi and Wang, Haibin
Journal: Molecular biology reports (2021): 21-31

Bottom-up modular synthesis of well-defined oligo(arylfuran)s.
Authors: Chen, Yang and Shen, Pingchuan and Cao, Tongxiang and Chen, Hao and Zhao, Zujin and Zhu, Shifa
Journal: Nature communications (2021): 6165

Page updated on November 19, 2024

Ordering information

Price
Unit size	10 Reactions 20 Reactions
Catalog Number	1748617487
Quantity

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Additional ordering information

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Spectral properties

Correction Factor (260 nm)	0.07
Correction Factor (280 nm)	0.073
Extinction coefficient (cm ^-1 M ^-1)	150000¹
Excitation (nm)	555
Emission (nm)	569
Quantum yield	0.15¹