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ReadiCleave™ FAM SSL-NHS ester
Fluorescence-based methods have many advantages for biological detections in terms of sensitivity and convenience. Many biological molecules can be readily labeled with a fluorescent tag for fluorescence imaging and flow cytometry analysis. However, most of the existing fluorescent tags are used to permanently labeling biological targets from which the added fluorescent tags cannot be cleaved for further downstream analysis, such as mass spectral analysis or another detection mode. AAT Bioquest’s ReadiCleave™ linkers enable fluorescent tags conjugated to a biological target from which the added fluorescent tag can be removed when needed. ReadiCleave™ FAM SSL-NHS ester contains a S-S linker that can be cleaved with TCEP or DTT to remove the fluorescein (FITC) fluorophore from the target molecule. 5-FAM has the same fluorophore identical to the common FITC dye. The cleavage can be carried out by adding DTT or TCEP solution and incubating from room temperature to 65 °C for 1-10 min.
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| ReadiCleave™ Cy5-SSL-NHS ester | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 |
| ReadiCleave™ XFD532 SSL-NHS ester | 534 | 553 | 81000 | 0.611 | 0.24 | 0.09 |
References
View all 28 references: Citation Explorer
Fluorescein CorneoGraphy (FCG): Use of a repurposed fluorescein imaging technique to objectively standardize corneal staining.
Authors: Soifer, Matias and Azar, Nadim S and Blanco, Ricardo and Mousa, Hazem M and Ghalibafan, Seyyedehfatemeh and Tovar, Arianna and Mettu, Priyatham S and Allingham, Michael J and Cousins, Scott W and Sabater, Alfonso L and Perez, Victor L
Journal: The ocular surface (2023): 77-79
Authors: Soifer, Matias and Azar, Nadim S and Blanco, Ricardo and Mousa, Hazem M and Ghalibafan, Seyyedehfatemeh and Tovar, Arianna and Mettu, Priyatham S and Allingham, Michael J and Cousins, Scott W and Sabater, Alfonso L and Perez, Victor L
Journal: The ocular surface (2023): 77-79
Image-Based Longitudinal Characterization of Corneal Wound to Understand the Role of Sphingosine-1-Phosphate.
Authors: Basu, Sandip K and Mandal, Nawajes
Journal: Methods in molecular biology (Clifton, N.J.) (2023): 337-345
Authors: Basu, Sandip K and Mandal, Nawajes
Journal: Methods in molecular biology (Clifton, N.J.) (2023): 337-345
The TGM2 inhibitor cysteamine hydrochloride does not impact corneal epithelial and stromal wound healing in vitro and in vivo.
Authors: Minella, A L and Casanova, M I and Chokshi, T J and Kang, J and Cosert, K and Gragg, M M and Bowman, M A and Mccorkell, M E and Daley, N L and Leonard, B C and Murphy, C J and Raghunathan, V K and Thomasy, S M
Journal: Experimental eye research (2023): 109338
Authors: Minella, A L and Casanova, M I and Chokshi, T J and Kang, J and Cosert, K and Gragg, M M and Bowman, M A and Mccorkell, M E and Daley, N L and Leonard, B C and Murphy, C J and Raghunathan, V K and Thomasy, S M
Journal: Experimental eye research (2023): 109338
Focused ultrasound stimulation on meibomian glands for the treatment of evaporative dry eye.
Authors: Lu, Gengxi and Gollapudi, Sumanth and Li, Runze and Pfeiffer, Margaret L and Mehta, Preeya and Jiang, Laiming and Hamm-Alvarez, Sarah and Humayun, Mark and Zhou, Qifa and Zhang-Nunes, Sandy X
Journal: Experimental biology and medicine (Maywood, N.J.) (2022): 519-526
Authors: Lu, Gengxi and Gollapudi, Sumanth and Li, Runze and Pfeiffer, Margaret L and Mehta, Preeya and Jiang, Laiming and Hamm-Alvarez, Sarah and Humayun, Mark and Zhou, Qifa and Zhang-Nunes, Sandy X
Journal: Experimental biology and medicine (Maywood, N.J.) (2022): 519-526
Human platelet lysate delivered via an ocular wound chamber for the treatment of corneal epithelial injuries.
Authors: Griffith, Gina L and Holt, Andrew W and Eriksson, Elof and Johnson, Anthony J and McDaniel, Jennifer S
Journal: Experimental eye research (2021): 108493
Authors: Griffith, Gina L and Holt, Andrew W and Eriksson, Elof and Johnson, Anthony J and McDaniel, Jennifer S
Journal: Experimental eye research (2021): 108493
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