ReadiCleave™ AML Cleavage Buffer 5X Aqueous Solution

Ordering information

Price
Catalog Number
Unit Size
Quantity

Add to cart

Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Solvent

Water

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Overview SDS Protocol

ReadiCleave™ AML Cleavage Buffer provides an optimized formula to effectively remove the fluorescence tags from our ReadiCleave™ AML-labeled biological targets when needed. The removal of the fluorescent ReadiCleave™ AML tags may make the fluorescence imaging more compatible with the downstream analysis of a biological target by other techniques, e.g., MS analysis. Using ReadiCleave™ AML Cleavage Buffer alongside ReadiCleave™ Styramides enables multiplexed fluorescence imaging applications in cells and tissues. ReadiCleave™ Styramides are the new iteration of our PSA products that add the reversible capability to chemically remove the PSA staining on tissue or in cells (if desired) while preserving the integrity of tissue samples.

Example protocol

PREPARATION OF WORKING SOLUTION

ReadiCleave™ AML Cleavage Buffer Working Solution (1X)

To prepare a 1X working solution, add 200 μL of ReadiCleave™ AML Cleavage Buffer into 800 μL of ddH2O, and mix thoroughly.

Note: For optimal results, use this solution within a few hours of preparation.

SAMPLE EXPERIMENTAL PROTOCOL

Remove the coverslip by briefly dipping the slides into PBS. Then, wash the tissue sample with PBS to eliminate any remaining mounting medium.
Add 100 µL of ReadiCleave™ AML Cleavage Buffer working solution to the tissue or cell samples.

Note: Add a sufficient amount of ReadiCleave™ AML Cleavage Buffer working solution to ensure that the samples are fully submerged.
Heat the samples at 60°C for 60 minutes.
Remove the ReadiCleave™ AML Cleavage Buffer working solution and briefly rinse the samples with PBST.
Reprocess the tissue samples beginning with the Antigen Retrieval step in your IHC staining protocol.

Images

Figure 1. Fluorescence imaging for the detection of EpCAM in lung adenocarcinoma was conducted using ReadiCleave™ iFluor® 488 Styramide (Cat# 45500), ReadiCleave™ iFluor® 546 Styramide (Cat# 45510), and ReadiCleave™ iFluor® 647 Styramide (Cat# 45520). Images were captured both before and after treatment with ReadiCleave™ AML Cleavage Buffer, providing comparative insights into the effectiveness of the cleavage buffer treatment.

References

View all 15 references: Citation Explorer

Synthesis and Antiproliferative Activity of 2,4,6,7-Tetrasubstituted-2H-pyrazolo[4,3-c]pyridines.
Authors: Razmienė, Beatričė and Řezníčková, Eva and Dambrauskienė, Vaida and Ostruszka, Radek and Kubala, Martin and Žukauskaitė, Asta and Kryštof, Vladimír and Šačkus, Algirdas and Arbačiauskienė, Eglė
Journal: Molecules (Basel, Switzerland) (2021)

Protein-drug conjugate programmed by pH-reversible linker for tumor hypoxia relief and enhanced cancer combination therapy.
Authors: Zhang, Xican and Feng, Liangzhu and Dong, Ziliang and Xin, Xiaoqian and Yang, Zhijuan and Deng, Dashi and Wagner, Ernst and Liu, Zhuang and Liu, Xiaowen
Journal: International journal of pharmaceutics (2020): 119321

Too Short-Lived or Not Existing Species: N-Azidoamines Reinvestigated.
Authors: Banert, Klaus and Pester, Tom
Journal: The Journal of organic chemistry (2019): 4033-4039

Iridium(iii) complex-based electrochemiluminescent probe for H2S.
Authors: Park, Joonho and Kim, Taemin and Kim, Hoon Jun and Hong, Jong-In
Journal: Dalton transactions (Cambridge, England : 2003) (2019): 4565-4573

Towards molecular electronic devices based on 'all-carbon' wires.
Authors: Moneo, Andrea and González-Orive, Alejandro and Bock, Sören and Fenero, Marta and Herrer, I Lucía and Milan, David C and Lorenzoni, Matteo and Nichols, Richard J and Cea, Pilar and Perez-Murano, Francesc and Low, Paul J and Martin, Santiago
Journal: Nanoscale (2018): 14128-14138

3-Fluoroazetidinecarboxylic Acids and trans,trans-3,4-Difluoroproline as Peptide Scaffolds: Inhibition of Pancreatic Cancer Cell Growth by a Fluoroazetidine Iminosugar.
Authors: Liu, Zilei and Jenkinson, Sarah F and Vermaas, Tom and Adachi, Isao and Wormald, Mark R and Hata, Yukako and Kurashima, Yukiko and Kaji, Akira and Yu, Chu-Yi and Kato, Atsushi and Fleet, George W J
Journal: The Journal of organic chemistry (2015): 4244-58

A gas phase cleavage reaction of cross-linked peptides for protein complex topology studies by peptide fragment fingerprinting from large sequence database.
Authors: Buncherd, Hansuk and Roseboom, Winfried and de Koning, Leo J and de Koster, Chris G and de Jong, Luitzen
Journal: Journal of proteomics (2014): 65-77

Emodin azide methyl anthraquinone derivative induced G0/ G1 arrest in HER2/neu-overexpressing MDA-MB-453 breast cancer cells.
Authors: Yan, Yan-yan and Fu, Li-wu and Zhang, Wei and Ma, Hong-shan and Ma, Cun-gen and Liang, Yong-ju and Liu, Bin-yu and Yu, Jie-zhong and Wu, Qiu-zhen and Dong, Yi-min
Journal: Journal of B.U.ON. : official journal of the Balkan Union of Oncology (2014): 650-5

Isolation of cross-linked peptides by diagonal strong cation exchange chromatography for protein complex topology studies by peptide fragment fingerprinting from large sequence databases.
Authors: Buncherd, Hansuk and Roseboom, Winfried and Ghavim, Behrad and Du, Weina and de Koning, Leo J and de Koster, Chris G and de Jong, Luitzen
Journal: Journal of chromatography. A (2014): 34-46

DNA sequencing by synthesis using 3'-O-azidomethyl nucleotide reversible terminators and surface-enhanced Raman spectroscopic detection.
Authors: Palla, Mirkó and Guo, Wenjing and Shi, Shundi and Li, Zengmin and Wu, Jian and Jockusch, Steffen and Guo, Cheng and Russo, James J and Turro, Nicholas J and Ju, Jingyue
Journal: RSC advances (2014): 49342-49346

Documents

Safety data sheet
Product protocol
Certificate of analysis

Location: Home / Products / ReadiCleave™ AML Cleavage Buffer *5X Aqueous Solution*

About Privacy Terms of Use Terms of Sales Distributors

ReadiCleave™ AML Cleavage Buffer *5X Aqueous Solution*

OverviewSDSProtocol