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ReadiCleave™ AML Cleavage Buffer *5X Aqueous Solution*
Example protocol
PREPARATION OF WORKING SOLUTION
To prepare a 1X working solution, add 200 μL of ReadiCleave™ AML Cleavage Buffer into 800 μL of ddH2O, and mix thoroughly.
Note: For optimal results, use this solution within a few hours of preparation.
SAMPLE EXPERIMENTAL PROTOCOL
Remove the coverslip by briefly dipping the slides into PBS. Then, wash the tissue sample with PBS to eliminate any remaining mounting medium.
Add 100 µL of ReadiCleave™ AML Cleavage Buffer working solution to the tissue or cell samples.
Note: Add a sufficient amount of ReadiCleave™ AML Cleavage Buffer working solution to ensure that the samples are fully submerged.
Heat the samples at 60°C for 60 minutes.
Remove the ReadiCleave™ AML Cleavage Buffer working solution and briefly rinse the samples with PBST.
Reprocess the tissue samples beginning with the Antigen Retrieval step in your IHC staining protocol.
References
Authors: Razmienė, Beatričė and Řezníčková, Eva and Dambrauskienė, Vaida and Ostruszka, Radek and Kubala, Martin and Žukauskaitė, Asta and Kryštof, Vladimír and Šačkus, Algirdas and Arbačiauskienė, Eglė
Journal: Molecules (Basel, Switzerland) (2021)
Authors: Zhang, Xican and Feng, Liangzhu and Dong, Ziliang and Xin, Xiaoqian and Yang, Zhijuan and Deng, Dashi and Wagner, Ernst and Liu, Zhuang and Liu, Xiaowen
Journal: International journal of pharmaceutics (2020): 119321
Authors: Banert, Klaus and Pester, Tom
Journal: The Journal of organic chemistry (2019): 4033-4039
Authors: Park, Joonho and Kim, Taemin and Kim, Hoon Jun and Hong, Jong-In
Journal: Dalton transactions (Cambridge, England : 2003) (2019): 4565-4573
Authors: Moneo, Andrea and González-Orive, Alejandro and Bock, Sören and Fenero, Marta and Herrer, I Lucía and Milan, David C and Lorenzoni, Matteo and Nichols, Richard J and Cea, Pilar and Perez-Murano, Francesc and Low, Paul J and Martin, Santiago
Journal: Nanoscale (2018): 14128-14138
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