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RatioWorks™ PH165
RatioWorks™ PH165 is a novel fluorescent probe designed for pH measurement in solution, featuring pH-dependent fluorescence spectra and unique dual excitation and dual emission properties. At low pH, RatioWorks™ PH165 is weakly fluorescent in the far-red spectrum region and highly fluorescent in the red spectrum region. As pH increases, it shows higher fluorescence in the far-red spectrum and lower fluorescence in the red spectrum. This probe quantifies pH in the 4 to 9 range, utilizing dual excitation/emission at 497/594 nm and 578/654 nm, respectively, and has a pKa of ~7.1. The red fluorescence spectrum of RatioWorks™ PH165 makes it suitable for multiplexing tests with green fluorophores like GFP. RatioWorks™ PH165 AM is cell-permeable and compatible with platforms such as fluorescence microscopy and microplate readers.
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) |
| RatioWorks™ PDMPO | 333 | 531 |
Citations
View all 3 citations: Citation Explorer
Intracellular pH Measurements in Glioblastoma Cells Using the pH-Sensitive Dye BCECF
Authors: Galifianakis, N. V., Placantonakis, D. G., Chesler, M.
Journal: Methods Mol Biol (2018): 103-109
Authors: Galifianakis, N. V., Placantonakis, D. G., Chesler, M.
Journal: Methods Mol Biol (2018): 103-109
Evaluation of BCECF fluorescence ratio imaging to properly measure gastric intramucosal pH variations in vivo
Authors: Rochon, P., Jourdain, M., Mangalaboyi, J., Fourrier, F., Soulie-Begu, S., Buys, B., Dehlin, G., Lesage, J. C., Chambrin, M. C., Mordon, S.
Journal: J Biomed Opt (2007): 064014
Authors: Rochon, P., Jourdain, M., Mangalaboyi, J., Fourrier, F., Soulie-Begu, S., Buys, B., Dehlin, G., Lesage, J. C., Chambrin, M. C., Mordon, S.
Journal: J Biomed Opt (2007): 064014
Calibration methods and avoidance of errors in measurement of intracellular pH (pHcyt) using the indicator bis(carboxyethyl)-5(6)-carboxyfluorescein (BCECF) in human platelets
Authors: Valant, P. A., Haynes, D. H.
Journal: J Fluoresc (1992): 191-206
Authors: Valant, P. A., Haynes, D. H.
Journal: J Fluoresc (1992): 191-206
References
View all 1 references: Citation Explorer
Estimation of intramitochondrial pca and ph by fura-2 and 2,7 biscarboxyethyl-5(6)-carboxyfluorescein (bcecf) fluorescence
Authors: Davis MH, Altschuld RA, Jung DW, Brierley GP.
Journal: Biochem Biophys Res Commun (1987): 40
Authors: Davis MH, Altschuld RA, Jung DW, Brierley GP.
Journal: Biochem Biophys Res Commun (1987): 40
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