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QXY21 [equivalent to QSY-21]

QXY21 is the same molecule to QSY-21 acid (ThermoFisher). QSY-21 has a broad and intense absorption at ~661 nm maxima with no detectable fluorescence, making it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications. It is a common long-wavelength quencher to best pair with Cy5, Alexa Fluor 647, iFluor 647 or other spectrally similar fluorescent dyes.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of QXY21 [equivalent to QSY-21] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM139.223 µL696.117 µL1.392 mL6.961 mL13.922 mL
5 mM27.845 µL139.223 µL278.447 µL1.392 mL2.784 mL
10 mM13.922 µL69.612 µL139.223 µL696.117 µL1.392 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

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Spectrum

Citations

View all 12 citations: Citation Explorer
A Sensitive Near-Infrared Fluorescent Sensor for Mitochondrial Hydrogen Sulfide
Authors: Ji, A., Fan, Y., Ren, W., Zhang, S., Ai, H. W.
Journal: ACS Sens (2018): 992-997
Imaging intracellular quantum dots: fluorescence microscopy and transmission electron microscopy
Authors: Szymanski, C. J., Yi, H., Liu, J. L., Wright, E. R., Payne, C. K.
Journal: Methods Mol Biol (2013): 21-33
Reversible off-on fluorescence probe for hypoxia and imaging of hypoxia-normoxia cycles in live cells
Authors: Takahashi, S., Piao, W., Matsumura, Y., Komatsu, T., Ueno, T., Terai, T., Kamachi, T., Kohno, M., Nagano, T., Hanaoka, K.
Journal: J Am Chem Soc (2012): 19588-91
Family of enhanced photoacoustic imaging agents for high-sensitivity and multiplexing studies in living mice
Authors: de la Zerda, A., Bodapati, S., Teed, R., May, S. Y., Tabakman, S. M., Liu, Z., Khuri-Yakub, B. T., Chen, X., Dai, H., Gambhir, S. S.
Journal: ACS Nano (2012): 4694-701
Visualizing mechanical tension across membrane receptors with a fluorescent sensor
Authors: Stabley, D. R., Jurchenko, C., Marshall, S. S., Salaita, K. S.
Journal: Nat Methods (2011): 64-7

References

View all 1 references: Citation Explorer
Synthesis and characterization of a small, membrane-permeant, caspase-activatable far-red fluorescent peptide for imaging apoptosis
Authors: Bullok K, Piwnica-Worms D.
Journal: J Med Chem (2005): 5404
Page updated on December 1, 2024

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Catalog Number2031
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Physical properties

Molecular weight

718.27

Solvent

DMSO

Spectral properties

Absorbance (nm)

660

Correction Factor (280 nm)

0.32

Extinction coefficient (cm -1 M -1)

890001

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200
In the presence of an activating agent (e.g., EDC, DCC etc) QSY-21 acid reacts with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.
In the presence of an activating agent (e.g., EDC, DCC etc) QSY-21 acid reacts with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.
In the presence of an activating agent (e.g., EDC, DCC etc) QSY-21 acid reacts with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.
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