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Quin-2, tetrapotassium salt *CAS 149022-19-5*
Quin-2 binds calcium tightly and resembles calcium chelator EGTA in ability to bind calcium much more tightly than magnesium. Binding of calcium causes large changes in ultraviolet absorption and fluorescence. The wavelengths of light that cause fluorescence when calcium is bound are longer than the wavelengths that cause fluorescence when it is not bound. When excited at two different wavelengths, the ratio of the fluorescence intensities at the two wavelengths gives the ratio of the concentrations of bound to free calcium. Free Quin-2 concentration can be measured precisely, so free calcium concentration can be calculated precisely. Quin-2 may be injected into cells to measure moment-to-moment changes in intracellular calcium concentration. Quin-2 AM is permeable to cells, and used for studying live cells.
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Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of Quin-2, tetrapotassium salt *CAS 149022-19-5* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 144.119 µL | 720.596 µL | 1.441 mL | 7.206 mL | 14.412 mL |
| 5 mM | 28.824 µL | 144.119 µL | 288.238 µL | 1.441 mL | 2.882 mL |
| 10 mM | 14.412 µL | 72.06 µL | 144.119 µL | 720.596 µL | 1.441 mL |
Molarity calculator
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References
View all 60 references: Citation Explorer
W-5 and quin 2-AM reverse the inhibitory effect of insulin on lipolysis due to dibutyryl cAMP
Authors: Goko H, Matsuoka A.
Journal: Diabetes Res Clin Pract (1999): 101
Authors: Goko H, Matsuoka A.
Journal: Diabetes Res Clin Pract (1999): 101
Calcium chelator Quin-2 prevents crocidolite-induced DNA strand breakage in human white blood cells
Authors: Faux SP, Michelangeli F, Levy LS.
Journal: Mutat Res (1994): 209
Authors: Faux SP, Michelangeli F, Levy LS.
Journal: Mutat Res (1994): 209
Fluorescence lifetime imaging of intracellular calcium in COS cells using Quin-2
Authors: Lakowicz JR, Szmacinski H, Nowaczyk K, Lederer WJ, Kirby MS, Johnson ML.
Journal: Cell Calcium (1994): 7
Authors: Lakowicz JR, Szmacinski H, Nowaczyk K, Lederer WJ, Kirby MS, Johnson ML.
Journal: Cell Calcium (1994): 7
Possible mechanisms of epinephrine actions in quin-2-loaded platelets refractory to arachidonic acid
Authors: Rao GH, Gerrard JM, Murthy M, White JG.
Journal: Biochem Med Metab Biol (1993): 322
Authors: Rao GH, Gerrard JM, Murthy M, White JG.
Journal: Biochem Med Metab Biol (1993): 322
Fluorescence lifetime imaging of calcium using Quin-2
Authors: Lakowicz JR, Szmacinski H, Nowaczyk K, Johnson ML.
Journal: Cell Calcium (1992): 131
Authors: Lakowicz JR, Szmacinski H, Nowaczyk K, Johnson ML.
Journal: Cell Calcium (1992): 131
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