Protonex™ Red 600-Dextran Conjugate *10,000 MW for Endocytosis*

• pH-sensitive, no-wash detection: Nonfluorescent at neutral pH but fluoresces bright red in acidic endocytic vesicles, eliminating the need for wash steps or external quencher dyes.
• Bright red fluorescence: Delivers high-intensity, high-contrast signals inside endosomes and lysosomes with minimal background, enabling clear visualization of internalized cargo.
• Multiplexing capability: Spectrally compatible with common green fluorophores (e.g., FITC, GFP) and other channels, allowing multi-color assays and simultaneous tracking of multiple parameters.
• Versatile applications: Compatible with live-cell fluorescence microscopy, flow cytometry, high-throughput screening (HTS), high-content analysis (HCA), and cell tracing studies.
• Direct Alternative to pHrodo™ Red Dextran: Matches or exceeds performance in phagocytosis assays and endocytosis imaging

Protonex™ Red 600-Dextran Conjugate *10,000 MW for Endocytosis* is a pH-sensitive red fluorescent dextran dye designed for real-time, wash-free imaging of endocytosis, phagocytosis, and lysosomal acidification in live-cell assays. Nonfluorescent at neutral pH, it emits a bright red fluorescent signal upon internalization into acidic compartments (e.g., endosomes and lysosomes), eliminating the need for secondary quencher dyes or disruptive wash steps.

Its Texas Red®-compatible spectral profile ensures seamless integration into existing fluorescence imaging platforms, including widefield and confocal microscopy, flow cytometry, and automated plate readers. Its spectral characteristics permit multiplexing with green (e.g., FITC, GFP) and far-red fluorophores, supporting multi-channel analysis in complex assay formats.

Designed to streamline workflows, Protonex™ Red 600-Dextran accelerates high-throughput screening (HTS) and high-content analysis (HCA) by delivering strong signal-to-background ratios while maintaining excellent cell viability. It is particularly well suited for applications such as antibody-dependent cellular phagocytosis (ADCP) assays in immuno-oncology, monitoring of autophagic flux, and tracing of neuronal or vascular structures. The consistent performance and minimal protocol requirements of Protonex™ Red 600-Dextran not only reduce variability and experimental complexity but facilitate reliable quantification of endocytic uptake, phagosome maturation, and intracellular trafficking dynamics in live-cell systems.

• Phagocytosis & Endocytosis Assays
  Monitor phagosome acidification in macrophages or neutrophils, detect fluid-phase macropinocytosis in tumor cells, and measure uptake via flow cytometry for real-time readouts.
• Lysosomal Tracking & Acidification Studies
  Quantify lysosomal pH changes in live cells, investigate endocytic trafficking and autophagy-related pathways, and capture acidification events without the need for wash steps.
• ADCP Assays (Antibody-Dependent Cellular Phagocytosis)
  Assess how immune cells engulf bacteria, beads, or labeled cancer cells, providing crucial insights for immunotherapy and drug development.
• High-Throughput & High-Content Screening
  Leverage streamlined, wash-free detection compatible with automated plate readers, robotic workflows, and confocal imaging to boost assay speed and scalability.
• Neuronal & Vascular Tracing
  Track dextrans across the blood-brain barrier or within neuronal networks, enabling detailed neuroanatomical and cell lineage studies that require precise intracellular trafficking data.
• Intracellular Communication & Cytoplasmic Analysis
  Investigate gap junction dynamics, cell–cell transfer, and cytoplasmic matrix properties (e.g., viscosity, diffusion) to uncover key cellular processes in diverse research areas.