Protonex™ Red 600

Protonex™ Red dye demonstrated pH-dependent fluorescence. Unlike most of the existing fluorescent dyes that are more fluorescent at higher pH, acidic conditions enhance the fluorescence of Protonex™ Red dye. The fluorescence of Protonex™ Red dye dramatically increases as pH decreases from neutral to the acidic. The weak fluorescence outside the cell may potentially eliminates the wash steps. Protonex™ Red dye provides a powerful tool to monitor acidic cell compartments such as endosomes and lysosomes. Protonex™ Red dye is weakly fluorescent outside the cells, but its fluorescence is significantly enhanced in acidic compartments (such as phagosomes, lysosomes and endosomes). This Protonex™ Red enables the specific detection of cellular acidic compartments with reduced signal variability and improved accuracy for imaging or flow applications. It can be also used for multiplexing cellular functional analysis with green dyes such as GFP, Fluo-8, calcein, or FITC-labeled antibodies. Protonex™ Red has the spectral properties similar to those of Texas Red, making the common filter set of Texas Red readily available to the assays of Protonex™ Red.

Page updated on November 21, 2024