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Protonex™ Lyso-NIR 770

Protonex™ Lyso-NIR 770 is a fluorescent derivative prepared from our Protonex™ NIR 770 NHS ester. It is cell-permeable and selectively localized in acidic organelles such as lysosomes. It is the only cell-permeable acidotropic pH dye with NIR fluorescence for monitoring pH changes in acidic organelles. Its fluorescence can be readily captured with the common Cy7 filter set that is equipped with most of the fluorescence instruments. Due to its significantly red-shifted excitation and emission wavelengths, it has much lower background than the existing pH sensors (such as Lysosensors). It might be used in vivo for monitoring pH changes in animals due to the significantly improved skin-penetration capability of NIR excitation.

Spectrum

References

View all 50 references: Citation Explorer
Rational design of a ratiometric fluorescent probe for imaging lysosomal nitroreductase activity.
Authors: Bai, Wenjun and Li, Yixuan and Zhao, Li and Li, Ruxin and Geng, Jiahou and Lu, Yang and Zhao, Yufen and Wang, Jinhui
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2023): 123032
A potent fluorescent transmembrane HCl transporter perturbs cellular pH and promotes cancer cell death.
Authors: Fares, Mohamed and Wu, Xin and McNaughton, Daniel A and Gilchrist, Alexander M and Lewis, William and Keller, Paul A and Arias-Betancur, Alain and Fontova, Pere and Pérez-Tomás, Ricardo and Gale, Philip A
Journal: Organic & biomolecular chemistry (2023): 2509-2515
Analysis of Radachlorin localization in living cells by fluorescence lifetime imaging microscopy.
Authors: Belashov, A V and Zhikhoreva, A A and Salova, A V and Belyaeva, T N and Litvinov, I K and Kornilova, E S and Semenova, I V and Vasyutinskii, O S
Journal: Journal of photochemistry and photobiology. B, Biology (2023): 112699
In vivo Assessment of Lysosomal Stress in the Drosophila Brain Using Confocal Fluorescence Microscopy.
Authors: Martelli, Felipe
Journal: Bio-protocol (2023): e4599
A water-soluble fluorescent pH probe and its application for monitoring lysosomal pH changes in living cells.
Authors: Zhang, Lei and Guo, Jun and You, Qihua and Xu, Yuqing
Journal: Analytical methods : advancing methods and applications (2023): 3057-3063
Page created on January 15, 2024, last updated on September 1, 2024

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Catalog Number21242
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Physical properties

Molecular weight

736.76

Solvent

DMSO

Spectral properties

Excitation (nm)

748

Emission (nm)

769

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
Image of Hela cells stained with Protonex™ Lyso-NIR 770. Hela cells immersed in a cell culture medium were loaded with 5 µM of Protonex™ Lyso-NIR 770 in HH buffer per well and incubated at 37⁰C for 30 min. The cells were then washed 3 times with HH buffer, and 100 µL of fresh cell culture media was added to each well. The images were captured in the Cy7 channel. Additionally, the cells were co-stained with LysoBrite™ Red (Cat# 22645), with images acquired in the TRITC channel but presented with a pseudo-Green Color. The overlay images reveal that Protonex™ Lyso-NIR 770 effectively stains the lysosome of HeLa cells.
Image of Hela cells stained with Protonex™ Lyso-NIR 770. Hela cells immersed in a cell culture medium were loaded with 5 µM of Protonex™ Lyso-NIR 770 in HH buffer per well and incubated at 37⁰C for 30 min. The cells were then washed 3 times with HH buffer, and 100 µL of fresh cell culture media was added to each well. The images were captured in the Cy7 channel. Additionally, the cells were co-stained with LysoBrite™ Red (Cat# 22645), with images acquired in the TRITC channel but presented with a pseudo-Green Color. The overlay images reveal that Protonex™ Lyso-NIR 770 effectively stains the lysosome of HeLa cells.
Image of Hela cells stained with Protonex™ Lyso-NIR 770. Hela cells immersed in a cell culture medium were loaded with 5 µM of Protonex™ Lyso-NIR 770 in HH buffer per well and incubated at 37⁰C for 30 min. The cells were then washed 3 times with HH buffer, and 100 µL of fresh cell culture media was added to each well. The images were captured in the Cy7 channel. Additionally, the cells were co-stained with LysoBrite™ Red (Cat# 22645), with images acquired in the TRITC channel but presented with a pseudo-Green Color. The overlay images reveal that Protonex™ Lyso-NIR 770 effectively stains the lysosome of HeLa cells.

Documents

pdfSafety data sheet
pdfProduct protocol
pdfCertificate of analysis
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