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ProLite™ FAST Blue Protein Gel Stain *200 Gels*
Example protocol
PREPARATION OF STOCK SOLUTIONS
Prepare a stock solution by adding 500 µL of DMSO into the vial of ProLite™ FAST Blue Protein Gel Stain (Component A).
Note: Any unused stock solution should be divided into single-use aliquots and stored at ≤-20 °C for two weeks. Avoid repeated freeze-thaw cycles and minimize light exposure.
Note: The 500 µL stock solution can effectively stain up to 200 gels, with each gel containing 10 wells.
PREPARATION OF WORKING SOLUTION
Prepare a working solution by adding 50 µL of the ProLite™ FAST Blue Protein Gel Stain stock solution to 1 mL of Staining buffer (Component B).
Note: Any unused working solution should be divided into single-use aliquots and stored at ≤-20 °C for two weeks. Avoid repeated freeze-thaw cycles and minimize light exposure.
SAMPLE EXPERIMENTAL PROTOCOL
Add 0.25 µL of the ProLite™ FAST Blue Protein Gel Stain stock solution to 10 µL of the protein lysate.
Note: The dye volume can be adjusted based on the total volume and protein concentration used in the lysate.
Incubate the mixture at room temperature for 15 to 60 minutes, protected from light.
Add the loading dye to the protein lysate as per the manufacturer's guidelines.
Load the samples onto the gel and run.
Following the completion of the gel run, the samples should be visibly present within the gel.
Safety data sheet