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PRO-TAO-3 [equivalent to TO-PRO-3]

PRO-TAO-3 is the same molecule to TO-PRO®-3 stain. TO-PRO®-3 and the other monomeric cyanine stains have wide applicability due to their low background and bright fluorescence. It is a carbocyanine monomer nucleic acid stain that can be well excited by a red laser at 633 nm or 647 nm with far-red fluorescence similar to Cy®5 dyes. It is useful as a nuclear counterstain and dead cell indicator. It is selective for detecting dsDNA (double-stranded nucleic acids). The cell-impermeable PRO-TTO-3 is widely used for flow cytometry and fluorescence microscopy as a long-wavelength fluorescence well separated from green and red fluorophores in multicolor applications. TO-PRO® is the trademark of ThermoFisher Scientific.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yield
TWO-PRO™ 3 [equivalent to TO-PRO®-3] *5 mM DMSO Solution* *CAS 157199-63-8*64265710200010.111

References

View all 50 references: Citation Explorer
Anti-inflammatory functions of the glucocorticoid receptor require DNA binding.
Authors: Escoter-Torres, Laura and Greulich, Franziska and Quagliarini, Fabiana and Wierer, Michael and Uhlenhaut, Nina Henriette
Journal: Nucleic acids research (2020)
Persistent DNA damage triggers activation of the integrated stress response to promote cell survival under nutrient restriction.
Authors: Clementi, Elena and Inglin, Larissa and Beebe, Erin and Gsell, Corina and Garajova, Zuzana and Markkanen, Enni
Journal: BMC biology (2020): 36
DNA methylation dynamics underlie metamorphic gene regulation programs in Xenopus tadpole brain.
Authors: Kyono, Yasuhiro and Raj, Samhitha and Sifuentes, Christopher J and Buisine, Nicolas and Sachs, Laurent and Denver, Robert J
Journal: Developmental biology (2020): 180-196
Mithramycin selectively attenuates DNA-damage-induced neuronal cell death.
Authors: Makarevich, Oleg and Sabirzhanov, Boris and Aubrecht, Taryn G and Glaser, Ethan P and Polster, Brian M and Henry, Rebecca J and Faden, Alan I and Stoica, Bogdan A
Journal: Cell death & disease (2020): 587
DNA-PKcs has KU-dependent function in rRNA processing and haematopoiesis.
Authors: Shao, Zhengping and Flynn, Ryan A and Crowe, Jennifer L and Zhu, Yimeng and Liang, Jialiang and Jiang, Wenxia and Aryan, Fardin and Aoude, Patrick and Bertozzi, Carolyn R and Estes, Verna M and Lee, Brian J and Bhagat, Govind and Zha, Shan and Calo, Eliezer
Journal: Nature (2020): 291-296
Page updated on July 12, 2023

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Catalog Number17565
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Physical properties

Molecular weight

671.42

Solvent

DMSO

Spectral properties

Extinction coefficient (cm -1 M -1)

102000

Excitation (nm)

642

Emission (nm)

657

Quantum yield

0.111

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501

CAS

157199-63-8
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