Portelite™ Fluorimetric Sodium Ion Quantification Kit
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Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Molecular weight | 720.72 |
Solvent | DMSO |
Excitation (nm) | 491 |
Emission (nm) | 511 |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Overview | ![]() ![]() |
Molecular weight 720.72 | Excitation (nm) 491 | Emission (nm) 511 |
Platform
Qubit Fluorometer
Excitation | 480 nm |
Emission | 530 nm |
Instrument specification(s) | 0.2 mL PCR tube |
CytoCite Fluorometer
Excitation | 480 nm |
Emission | 530 nm |
Instrument specification(s) | 0.2 mL PCR tube |
Components
Example protocol
AT A GLANCE
Thaw all the kit components at room temperature before starting the experiment.
Prepare the test samples and serially diluted sodium standards.
Add 100 µL of the SoNa™ 520 working solution to your test samples.
Incubate at room temperature for 5-10 minutes.
Monitor the fluorescence intensity with a CytoCite™ fluorometer or Qubit™ fluorometer.
PREPARATION OF STOCK SOLUTIONS
Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles
To prepare a SoNa™ 520 stock solution, add 100 μL of DMSO to the vial containing SoNa™ 520 (Component A).
Note: Prepare a single aliquot of unused SoNa™ 520 stock solution and store it at ≤ -20 ºC, protected from light. Avoid freeze/thaw cycles.
PREPARATION OF STANDARD SOLUTIONS
https://www.aatbio.com/tools/serial-dilution/21327
PREPARATION OF WORKING SOLUTION
Prepare the SoNa™ 520 working solution by adding 100 μL of SoNa™ 520 (Component A) to 5 mL of Assay Buffer (Component B), and protect the working solution from light by covering it with foil or placing it in a dark location.
Note: For optimal results, use this solution within a few hours after preparing it.
Note: 5 mL of working solution is enough for 100 tests.
SAMPLE EXPERIMENTAL PROTOCOL
The acceptable sample volume can range from 1 to 20 μL, depending on the estimated concentration of the nucleic acid sample. The following protocol is based on a sample volume of 10 μL.
Add 100 µL of SoNa™ 520 working solution to each Cytocite™ sample tube (Cat No. CCT100) or to an equivalent 0.2 mL PCR tube.
Add 100 μL of Sodium Standards or test samples to each tube. Mix each tube by vortexing for 2-3 seconds.
Incubate all the tubes at room temperature for 5-10 minutes.
Insert the samples into either the CytoCite™ or Qubit™ devices. Use the green fluorescence channel to measure the fluorescence intensity. Be sure to follow the specific procedures for the CytoCite™ Fluorometer. For detailed instructions, refer to the link below:
https://devices.aatbio.com/documentation/user-manual-for-cytocite-fluorometer
For Portelite™ assays, you can create a calibration curve using the Sodium Standards. Below is a simple protocol for generating a customized Sodium standard curve.
Perform a 1:2 serial dilution. First, add Sodium Standard #2 (Component D) into the Assay Buffer (Component B). Then, create the following Sodium standard dilutions: 40 mM, 20 mM, 10 mM, 5 mM, 2.5 mM, 1.25 mM, and 0.625 mM.
Note: Final, in well concentration of the sample, will be 20, 10, 5, 2.5, 1.25, 0.625, 0.3125 mM.
Add 100 µL of the SoNa™ 520 working solution to each tube.
Add 100 µL of either standards or samples into a 0.2 mL PCR tube.
Incubate the reaction at room temperature for 5-10 minutes.
Insert the samples into the CytoCite™ device and use the green fluorescence channel to monitor the fluorescence intensity.
Calculators
Common stock solution preparation
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 138.75 µL | 693.751 µL | 1.388 mL | 6.938 mL | 13.875 mL |
5 mM | 27.75 µL | 138.75 µL | 277.5 µL | 1.388 mL | 2.775 mL |
10 mM | 13.875 µL | 69.375 µL | 138.75 µL | 693.751 µL | 1.388 mL |
Molarity calculator
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
/ | = | x | = |
Product Family
Name | Excitation (nm) | Emission (nm) |
Portelite™ Fluorimetric Lithium Ion Quantification Kit | 491 | 513 |
Amplite® Fluorimetric Sodium Ion Quantification Kit | 491 | 511 |
Images
References
Authors: Kushi, Efrem Negash and Belachew, Tefera and Tamiru, Dessalegn
Journal: Journal of nutritional science (2023): e5
Authors: Jiménez Rodríguez, Amelia and Palomo Cobos, Luis and Rodríguez-Martín, Amelia and Fernández Del Valle, Patricia and Novalbos-Ruíz, José P
Journal: Nutrients (2023)
Authors: Vera, Michael A and Sutphin, Angela and Hansen, Lisa and El-Khoury, Joe M
Journal: Laboratory medicine (2022): e105-e108
Authors: Evenepoel, A and Herroelen, P and Lanckmans, K and van der Werff Ten Bosch, J and Martin, M and Weets, I and van Dalem, A
Journal: Acta clinica Belgica (2022): 832-836
Authors: Ouatu, Anca and Mihai, Madalina Stefana and Tanase, Daniela Maria and Dascalu, Cristina Gena and Dima, Nicoleta and Serban, Lacramioara Ionela and Rezus, Ciprian and Floria, Mariana
Journal: Diagnostics (Basel, Switzerland) (2021)
Authors: Jia, Mengtian and Zhu, Yanyan and Guo, Dong and Bi, Xiaogang and Hou, Xiaohong
Journal: Analytica chimica acta (2020): 211-220
Authors: Tischer, Bruna and Teixeira, Iberê Damê and Filoda, Paula Freitas and Alessio, Keiti Oliveira and Barin, Juliano Smanioto and Duarte, Fábio Andrei and Kipper, Liane Mahlmann and Helfer, Gilson Augusto and Costa, Adilson Ben da
Journal: Food chemistry (2020): 125456
Authors: Barkhuizen, Marizna and Hoffmann, Mariza and Zöllner, Ekkehard Wa and Erasmus, Rajiv T and Zemlin, Annalise E
Journal: Biochemia medica (2019): 011002
Authors: Timmons, Andrew K and Korpak, Anna M and Tan, Jenny and Moore, Kathryn P and Liu, Cindy H and Forsberg, Christopher W and Goldberg, Jack and Smith, Nicholas L and Cohen, David M
Journal: American journal of physiology. Renal physiology (2019): F1114-F1123
Authors: Gazulla, María Fernanda and Andreu, Cristina and Rodrigo, Marta and Orduña, Mónica and Ventura, María Jesús
Journal: Talanta (2018): 600-605
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