Portelite™ Fluorimetric High Sensitivity DNA Quantitation Kit *Optimized for CytoCite™ and Qubit™ Fluorometers*

1. Prepare Helixyte™ Green working solution.

2. Add 190 µL 1X Helixyte Green™ working solution into each 0.2 mL PCR tube (Cat#: CCT100).

3. Add 10 µL DNA standards or test samples into each tube.

4. Incubate at room temperature for 2 minutes.

5. Monitor fluorescence with CytoCite™ fluorometer or Qubit™ fluorometer.

Bring kit components to room temperature before starting the experiment.

1. To prepare enough working solution for 10 samples, add 10 μL of Helixyte Green™ (Component A) to 2 mL of DNA Assay Buffer (Component B).

   Note: Protect the working solution from light by covering it with foil or placing it in the dark.

   Note: We recommend preparing this solution in a plastic container rather than glass, as the dye may adsorb to glass surfaces. For best results, this solution should be used within a few hours of its preparation.

The acceptable sample volume could range from 1~20 µL depending on the estimated concentration of the DNA sample. The recommended sample volume is 10 µL with the DNA concentration in 0.5~10 ng/ µL range. If another sample volume is being used, please adjust the dilution factor in the concentration calculations. 

1. Add 190 µL of 1X Helixyte Green™ working solution to each Cytocite™ sample tube (#CCT100) or an equivalent 0.2 mL PCR tube.

   Note: Use thin-wall, polypropylene, clear 0.2 mL PCR tubes such as #CCT100.

2. Add DNA standards or test samples 10 µL into each tube, and then mix by vortexing 2~3 seconds.

3. Allow all tubes to incubate at room temperature for 2 minutes.

4. Insert the samples into CytoCite™ or Quibit™ and monitor the fluorescence with a green fluorescence channel. Follow the procedure appropriate for CytoCite™ Fluorometer.

   Note: See the link below for detailed instructions:

   https://devices.aatbio.com/documentation/user-manual-for-cytocite-fluorometer

1. Perform dilution with DNA Assay Buffer to get 10, 8, 6, 4, 2, 1, 0.5, 0 ng/µL DNA standard dilutions.

2. Add 190 µL of Helixyte Green™ working solution into a 0.2 mL PCR tube.

3. Add 10 µL standards or 10 µL samples into each tube.

4. Incubate the reaction at room temperature for 2 minutes.

5. Insert the samples into CytoCite™ and monitor the fluorescence with a green fluorescence channel.