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pNPP [4-Nitrophenyl phosphate, disodium salt] *CAS 333338-18-4*
para-Nitrophenylphosphate (pNPP) is a chromogenic substrate for acid and alkaline phosphatase in ELISA assays. In the presence of phosphatases pNPP is hydrolyzed to generate the yellow para-nitrophenol that can be measured at 405 nm with an absorption microplate reader or a spectrophotometer.
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Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of pNPP [4-Nitrophenyl phosphate, disodium salt] *CAS 333338-18-4* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 269.44 µL | 1.347 mL | 2.694 mL | 13.472 mL | 26.944 mL |
| 5 mM | 53.888 µL | 269.44 µL | 538.88 µL | 2.694 mL | 5.389 mL |
| 10 mM | 26.944 µL | 134.72 µL | 269.44 µL | 1.347 mL | 2.694 mL |
Molarity calculator
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References
View all 52 references: Citation Explorer
Reference Interval of Serum Heat-stable Alkaline Phosphatase Activity in Pregnant Women in Zaria
Authors: Aliyu IS, Isah HS, Afonja OA.
Journal: Niger Postgrad Med J (2006): 31
Authors: Aliyu IS, Isah HS, Afonja OA.
Journal: Niger Postgrad Med J (2006): 31
Development of immunoassays for serum tartrate-resistant acid phosphatase isoform 5a
Authors: Chao TY, Lee SH, Chen MM, Neustadt DH, Chaudhry UA, Yam LT, Janckila AJ.
Journal: Clin Chim Acta (2005): 132
Authors: Chao TY, Lee SH, Chen MM, Neustadt DH, Chaudhry UA, Yam LT, Janckila AJ.
Journal: Clin Chim Acta (2005): 132
Alternative immunoassay for tartrate-resistant acid phosphatase isoform 5b using the fluorogenic substrate naphthol ASBI-phosphate and heparin
Authors: Janckila AJ, Simons RM, Yam LT.
Journal: Clin Chim Acta (2004): 157
Authors: Janckila AJ, Simons RM, Yam LT.
Journal: Clin Chim Acta (2004): 157
The N-terminal domain of mammalian soluble epoxide hydrolase is a phosphatase
Authors: Cronin A, Mowbray S, Durk H, Homburg S, Fleming I, Fisslthaler B, Oesch F, Ar and M., undefined
Journal: Proc Natl Acad Sci U S A (2003): 1552
Authors: Cronin A, Mowbray S, Durk H, Homburg S, Fleming I, Fisslthaler B, Oesch F, Ar and M., undefined
Journal: Proc Natl Acad Sci U S A (2003): 1552
Extracellular alkaline phosphatase from the filamentous fungus Aspergillus caespitosus: purification and biochemical characterization
Authors: Guimaraes LH, Terenzi HF, Jorge JA, Leone FA, Polizeli ML.
Journal: Folia Microbiol (Praha) (2003): 627
Authors: Guimaraes LH, Terenzi HF, Jorge JA, Leone FA, Polizeli ML.
Journal: Folia Microbiol (Praha) (2003): 627
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