pNPP [4-Nitrophenyl phosphate, disodium salt] *CAS 333338-18-4*
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Physical properties
Molecular weight | 371.14 |
Solvent | Water |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | ![]() ![]() |
See also: Alkaline Phosphatase (ALP), Biotin and Streptavidin, Enzyme-Linked Immunosorbent Assay (ELISA), Secondary Antibodies
CAS 333338-18-4 | Molecular weight 371.14 |
para-Nitrophenylphosphate (pNPP) is a chromogenic substrate for acid and alkaline phosphatase in ELISA assays. In the presence of phosphatases pNPP is hydrolyzed to generate the yellow para-nitrophenol that can be measured at 405 nm with an absorption microplate reader or a spectrophotometer.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of pNPP [4-Nitrophenyl phosphate, disodium salt] *CAS 333338-18-4* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 269.44 µL | 1.347 mL | 2.694 mL | 13.472 mL | 26.944 mL |
5 mM | 53.888 µL | 269.44 µL | 538.88 µL | 2.694 mL | 5.389 mL |
10 mM | 26.944 µL | 134.72 µL | 269.44 µL | 1.347 mL | 2.694 mL |
Molarity calculator
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References
View all 52 references: Citation Explorer
Reference Interval of Serum Heat-stable Alkaline Phosphatase Activity in Pregnant Women in Zaria
Authors: Aliyu IS, Isah HS, Afonja OA.
Journal: Niger Postgrad Med J (2006): 31
Authors: Aliyu IS, Isah HS, Afonja OA.
Journal: Niger Postgrad Med J (2006): 31
Development of immunoassays for serum tartrate-resistant acid phosphatase isoform 5a
Authors: Chao TY, Lee SH, Chen MM, Neustadt DH, Chaudhry UA, Yam LT, Janckila AJ.
Journal: Clin Chim Acta (2005): 132
Authors: Chao TY, Lee SH, Chen MM, Neustadt DH, Chaudhry UA, Yam LT, Janckila AJ.
Journal: Clin Chim Acta (2005): 132
Alternative immunoassay for tartrate-resistant acid phosphatase isoform 5b using the fluorogenic substrate naphthol ASBI-phosphate and heparin
Authors: Janckila AJ, Simons RM, Yam LT.
Journal: Clin Chim Acta (2004): 157
Authors: Janckila AJ, Simons RM, Yam LT.
Journal: Clin Chim Acta (2004): 157
The N-terminal domain of mammalian soluble epoxide hydrolase is a phosphatase
Authors: Cronin A, Mowbray S, Durk H, Homburg S, Fleming I, Fisslthaler B, Oesch F, Ar and M., undefined
Journal: Proc Natl Acad Sci U S A (2003): 1552
Authors: Cronin A, Mowbray S, Durk H, Homburg S, Fleming I, Fisslthaler B, Oesch F, Ar and M., undefined
Journal: Proc Natl Acad Sci U S A (2003): 1552
Extracellular alkaline phosphatase from the filamentous fungus Aspergillus caespitosus: purification and biochemical characterization
Authors: Guimaraes LH, Terenzi HF, Jorge JA, Leone FA, Polizeli ML.
Journal: Folia Microbiol (Praha) (2003): 627
Authors: Guimaraes LH, Terenzi HF, Jorge JA, Leone FA, Polizeli ML.
Journal: Folia Microbiol (Praha) (2003): 627
Chemiluminescent assay of alkaline phosphatase using dihydroxyacetone phosphate as substrate detected with lucigenin
Authors: Kokado A, Arakawa H, Maeda M.
Journal: Luminescence (2002): 5
Authors: Kokado A, Arakawa H, Maeda M.
Journal: Luminescence (2002): 5
A heat-sensitive Arabidopsis thaliana kinase substitutes for human p70s6k function in vivo
Authors: Turck F, Kozma SC, Thomas G, Nagy F.
Journal: Mol Cell Biol (1998): 2038
Authors: Turck F, Kozma SC, Thomas G, Nagy F.
Journal: Mol Cell Biol (1998): 2038
Partially folded conformations of inositol monophosphatase endowed with catalytic activity
Authors: Lau CK, Lo SC, Li W, Churchich DR, Kwok F, Churchich JE.
Journal: J Protein Chem (1998): 789
Authors: Lau CK, Lo SC, Li W, Churchich DR, Kwok F, Churchich JE.
Journal: J Protein Chem (1998): 789
Topological studies on the enzymes catalyzing the biosynthesis of Glc-P-dolichol and the triglucosyl cap of Glc3Man9GlcNAc2-P-P-dolichol in microsomal vesicles from pig brain: use of the processing glucosidases I/II as latency markers
Authors: Rush JS, Waechter CJ.
Journal: Glycobiology (1998): 1207
Authors: Rush JS, Waechter CJ.
Journal: Glycobiology (1998): 1207
Solvent kinetic isotope effects of human placental alkaline phosphatase in reverse micelles
Authors: Huang TM, Hung HC, Chang TC, Chang GG.
Journal: Biochem J (1998): 267
Authors: Huang TM, Hung HC, Chang TC, Chang GG.
Journal: Biochem J (1998): 267
Application notes
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