PhosphoWorks™ Fluorimetric Pyrophosphate Assay Kit *Enhanced Selectivity*

PhosphoWorks™ Fluorimetric Pyrophosphate Assay Kit

Enhanced Selectivity

Pyrophosphate (PPi) is produced by a number of biochemical reactions, such as ATP hydrolysis, DNA and RNA polymerizations, cyclic AMP formation by the enzyme adenylate cyclase and the enzymatic activation of fatty acids to form their coenzyme A esters. Our PhosphoWorks™ Pyrophosphate Assay Kit provides the most robust spectrophotometric method for measuring pyrophosphate. This kit uses our proprietary fluorogenic pyrophosphate sensor that has its fluorescence intensity proportionally dependent upon the concentration of pyrophosphate. The PPi sensor used in the kit has quite high selectivity to PPi compared to phosphate and ATP. Our assay is much easier and more robust than the enzyme-coupling pyrophosphate methods that require at least two enzymes for their pyrophosphate detections. The kit provides all the essential components for assaying pyrophosphate. This kit has been successfully used in high throughput screening (HTS). Please inquire special HTS bulk package discount for the screening of >10,000 assays.

Pyrophosphate, ATP and phosphate dose responses were measured with PhosphoWorks™ Fluoremetric Pyrophosphate Assay Kit in a solid black 96-well plate using a fluorescence microplate reader.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
21614	200 Tests	Price

Citations

View all 12 citations: Citation Explorer

Modulation of host ATP levels by secreted bacterial effectors

Authors:

He, Chunlin and Li, Chuang and Liu, Yao and Chen, Tao-Tao and Li, Chunxiuli and Chu, Xiao and Liu, Shuxin and Wang, Lidong and Zhang, Yong and Ouyang, Songying and others,

Journal:

Nature Communications (2025): 1--15

Inorganic Pyrophosphate at Serum Concentration May Not Be Able to Inhibit Mineralization: A Study in Aqueous Solutions and Serum

Authors:

Cheng, Yuxuan and Ru, Jing and Feng, Chaobo and Liu, Xiaohao and Zeng, Hua and Tan, Shuo and Chen, Xi and Chen, Feng and Lu, Bing-Qiang

Journal:

ACS Omega (2024)

Metabolic enzyme UAP1 mediates IRF3 pyrophosphorylation to facilitate innate immune response

Authors:

Yang, Shuai and Jin, Shouheng and Xian, Huifang and Zhao, Zhiyao and Wang, Liqiu and Wu, Yaoxing and Zhou, Liang and Li, Mengqiu and Cui, Jun

Journal:

Molecular Cell (2023)

AaTAS1 and AaMFS1 Genes for Biosynthesis or Efflux Transport of Tenuazonic Acid and Pathogenicity of Alternaria alternata

Authors:

Sun, Fan and Cao, Xueqiang and Yu, Dianzhen and Hu, Dongqiang and Yan, Zheng and Fan, Yingying and Wang, Cheng and Wu, Aibo

Journal:

Molecular Plant-Microbe Interactions (2022): 416--427

Biological studies and target engagement of the 2-C-methyl-d-erythritol 4-phosphate cytidylyltransferase (IspD)-targeting antimalarial agent (1 R, 3 S)-MMV008138 and analogs

Authors:

Ghavami, Maryam and Merino, Emilio F and Yao, Zhong-Ke and Elahi, Rubayet and Simpson, Morgan E and Fern{\'a}ndez-Murga, Maria L and Butler, Joshua H and Casasanta, Michael A and Krai, Priscilla M and Totrov, Maxim M and others,

Journal:

ACS infectious diseases (2017): 549--559

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12352200

Instrument settings

Fluorescence microplate reader
Excitation	370 nm
Emission	470 nm
Cutoff	455 nm
Recommended plate	Solid black

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

Telephone
Fax
Email	sales@aatbio.com
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Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
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Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 24, 2025

Component A: Assay Buffer	1 bottle (25 mL)
Component B: PPi Sensor	1 vial (lyophilized powder)
Component C: Pyrophosphate Standard	1 vial (1 mL, 50 mM)
Component D: DMSO	1 vial (100 µL)