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Phenol Red, sodium salt *CAS 34487-61-6*
Phenol red (also known as phenolsulfonphthalein or PSP) is a pH indicator that is frequently used in cell biology laboratories. Its color exhibits a gradual transition from yellow to red over the pH range 6.6 to 8.0. Above pH 8.1, phenol red turns a bright pink (fuschia) color. When cells are grown in tissue culture, the medium in which they grow is held close to the physiological pH. A small amount of phenol red added to the growth media will have a pink-red color under normal conditions. In the event of problems, waste products produced by dying cells or overgrowth of contaminants will cause a change in pH, leading to a change in indicator color.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of Phenol Red, sodium salt *CAS 34487-61-6* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 265.703 µL | 1.329 mL | 2.657 mL | 13.285 mL | 26.57 mL |
| 5 mM | 53.141 µL | 265.703 µL | 531.406 µL | 2.657 mL | 5.314 mL |
| 10 mM | 26.57 µL | 132.852 µL | 265.703 µL | 1.329 mL | 2.657 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Product family
| Name | Excitation (nm) | Emission (nm) |
| Calcein Red™ sodium salt | 562 | 576 |
References
View all 106 references: Citation Explorer
Effect of trypan blue staining on the density and viability of lens epithelial cells in white cataract
Authors: Nanavaty MA, Johar K, Sivasankaran MA, Vasavada AR, Praveen MR, Zetterstrom C.
Journal: J Cataract Refract Surg (2006): 1483
Authors: Nanavaty MA, Johar K, Sivasankaran MA, Vasavada AR, Praveen MR, Zetterstrom C.
Journal: J Cataract Refract Surg (2006): 1483
A prospective study on trypan blue capsule staining under air vs under viscoelastic
Authors: Wong VW, Lai TY, Lee GK, Lam PT, Lam DS.
Journal: Eye (2006): 820
Authors: Wong VW, Lai TY, Lee GK, Lam PT, Lam DS.
Journal: Eye (2006): 820
Hypothermia of 8 degrees C protects cultured retinal pigment epithelial cells and retinal ganglion cells against trypan blue toxicity
Authors: Kunikata H, Abe T, Murata H, Sagara Y, Wakusawa R, Sato H, Yoshida M, Fuse N, Tamai M.
Journal: Am J Ophthalmol (2006): 754
Authors: Kunikata H, Abe T, Murata H, Sagara Y, Wakusawa R, Sato H, Yoshida M, Fuse N, Tamai M.
Journal: Am J Ophthalmol (2006): 754
Toxic effects of indocyanine green, infracyanine green, and trypan blue on the human retinal pigmented epithelium
Authors: Kodjikian L, Richter T, Halberstadt M, Beby F, Flueckiger F, Boehnke M, Garweg JG.
Journal: Graefes Arch Clin Exp Ophthalmol (2005): 917
Authors: Kodjikian L, Richter T, Halberstadt M, Beby F, Flueckiger F, Boehnke M, Garweg JG.
Journal: Graefes Arch Clin Exp Ophthalmol (2005): 917
Trypan blue: effect on retinal pigment epithelial and neurosensory retinal cells
Authors: Narayanan R, Kenney MC, Kamjoo S, Trinh TH, Seigel GM, Resende GP, Kuppermann BD.
Journal: Invest Ophthalmol Vis Sci (2005): 304
Authors: Narayanan R, Kenney MC, Kamjoo S, Trinh TH, Seigel GM, Resende GP, Kuppermann BD.
Journal: Invest Ophthalmol Vis Sci (2005): 304
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