PerCP-iFluor® 700

PerCP (Peridinin-chlorophyll-protein complex) is isolated from Dinophyceae sp. It has an extremely high extinction coefficient, a high quantum efficiency and a large Stokes shift. It is well excited with the Argon laser at 488 nm with its maximum emission peak at 677 nm. PerCP protein is commonly used for fluorescent immunolabeling, particularly in applications involving fluorescent-activated cell sorting (FACS). PerCP-iFluor® 700 tandem conjugates can be excited with a standard 488 nm laser and emits in the far red at a longer wavelength for multicolor flow cytometric analysis of cells. These multiple emission wavelengths make PerCP-iFluor® 700 conjugates potentially useful fluorochromes for multicolor analysis with FITC, PE and other fluorochromes. PerCP tandem structure may make it more photostable than PerCP alone, which generally photobleaches rapidly with more powerful water-cooled gas lasers.

Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)	Correction Factor (565 nm)	Correction Factor (650 nm)
PerCP-iFluor® 710	719	747	350000	-	-	-	-	-
PerCP-iFluor® 780	778	806	350000	-	-	-	-	-
iFluor® 700 maleimide	690	713	220000¹	0.23¹	0.09	0.04	0.0766	0.4004
iFluor® 700 amine	690	713	220000¹	0.23¹	0.09	0.04	0.0766	0.4004
iFluor® 700 hydrazide	690	713	220000¹	0.23¹	0.09	0.04	0.0766	0.4004
iFluor® 700 Styramide Superior Replacement for Alexa Fluor 700 tyramide	690	713	220000¹	0.23¹	0.09	0.04	0.0766	0.4004

References

View all 50 references: Citation Explorer

Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis.
Authors: Zhang, Linlin and Liu, Mengjia and Zhang, Meng and Ai, Junhong and Tian, Jiao and Wang, Ran and Xie, Zhengde
Journal: Journal of visualized experiments : JoVE (2022)

Recombinant thrombomodulin attenuates hyper-inflammation and glycocalyx damage in a murine model of Streptococcus pneumoniae-induced sepsis.
Authors: Watanabe, Eizo and Akamatsu, Toshinobu and Ohmori, Masaaki and Kato, Mayu and Takeuchi, Noriko and Ishiwada, Naruhiko and Nishimura, Rintaro and Hishiki, Haruka and Fujimura, Lisa and Ito, Chizuru and Hatano, Masahiko
Journal: Cytokine (2022): 155723

Routine flow cytometry approach for the evaluation of solid tumor neoplasms and immune cells in minimally invasive samples.
Authors: Quirós-Caso, Covadonga and Arias Fernández, Tamara and Fonseca-Mourelle, Ariana and Torres, Héctor and Fernández, Luis and Moreno-Rodríguez, Maria and Ariza-Prota, Miguel Ángel and López-González, Francisco Julián and Carvajal-Álvarez, Miguel and Alonso-Álvarez, Sara and Moro-García, Marco Antonio and Colado, Enrique
Journal: Cytometry. Part B, Clinical cytometry (2022)

Platelet and neutrophil responses to Porphyromonas gingivalis in human whole blood.
Authors: Chen, William A and Fletcher, Hansel M and Payne, Kimberly J and Aka, Sheryl and Thornburg, Melanie B and Gheorghe, Joseph D and Safi, Shahnaj Binte and Shavlik, David and Oyoyo, Udochukwu and Boskovic, Danilo S
Journal: Molecular oral microbiology (2021): 202-213

The ISCCA flow protocol for the monitoring of anti-CD20 therapies in autoimmune disorders.
Authors: Gatti, Arianna and Buccisano, Francesco and Scupoli, Maria T and Brando, Bruno
Journal: Cytometry. Part B, Clinical cytometry (2021): 194-205

Page updated on December 17, 2024

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