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PE-Biotin Conjugate
The bifunctional PE-Biotin conjugate demonstrates excellent avidin binding and strong fluorescence upon binding to a protein or nucleic acid target. It has avidin-binding properties similar to native biotin in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers. PE-Biotin conjugate can be used for detecting and quantifying biotin binding sites of avidin, streptavidin or neutravidin. PE (R-Phycoerythrin), isolated from red algae, might be the brightest fluorophore. Its primary absorption peak is at 565 nm with secondary peaks at 496 and 545 nm. PE is the most intensely fluorescent phycobiliprotein, with quantum efficiencies probably in excess of 90%, and its orange fluorescence is readily visible by eye in any moderately concentrated solution.
Spectrum
References
View all 37 references: Citation Explorer
Comparison of Biosensing Methods Based on Different Isothermal Amplification Strategies: A Case Study with Erwinia amylovora.
Authors: Ivanov, Aleksandr V and Safenkova, Irina V and Drenova, Natalia V and Zherdev, Anatoly V and Dzantiev, Boris B
Journal: Biosensors (2022)
Authors: Ivanov, Aleksandr V and Safenkova, Irina V and Drenova, Natalia V and Zherdev, Anatoly V and Dzantiev, Boris B
Journal: Biosensors (2022)
Monitoring non-specific adsorption at solid-liquid interfaces by supercritical angle fluorescence microscopy.
Authors: Au, Aaron and Ho, Man and Wheeler, Aaron R and Yip, Christopher M
Journal: The Review of scientific instruments (2022): 113707
Authors: Au, Aaron and Ho, Man and Wheeler, Aaron R and Yip, Christopher M
Journal: The Review of scientific instruments (2022): 113707
Isolation of Cowpea Mosaic Virus-Binding Peptides.
Authors: Chan, Soo Khim and Steinmetz, Nicole F
Journal: Biomacromolecules (2021): 3613-3623
Authors: Chan, Soo Khim and Steinmetz, Nicole F
Journal: Biomacromolecules (2021): 3613-3623
A Plug-and-Play Platform for the Formation of Trifunctional Cysteine Bioconjugates that also Offers Control over Thiol Cleavability.
Authors: Bahou, Calise and Szijj, Peter A and Spears, Richard J and Wall, Archie and Javaid, Faiza and Sattikar, Afrah and Love, Elizabeth A and Baker, James R and Chudasama, Vijay
Journal: Bioconjugate chemistry (2021): 672-679
Authors: Bahou, Calise and Szijj, Peter A and Spears, Richard J and Wall, Archie and Javaid, Faiza and Sattikar, Afrah and Love, Elizabeth A and Baker, James R and Chudasama, Vijay
Journal: Bioconjugate chemistry (2021): 672-679
Nucleic acid lateral flow assay with recombinase polymerase amplification: Solutions for highly sensitive detection of RNA virus.
Authors: Ivanov, Aleksandr V and Safenkova, Irina V and Zherdev, Anatoly V and Dzantiev, Boris B
Journal: Talanta (2020): 120616
Authors: Ivanov, Aleksandr V and Safenkova, Irina V and Zherdev, Anatoly V and Dzantiev, Boris B
Journal: Talanta (2020): 120616
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