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Oxazine 1 *CAS 24796-94-9*
Oxazine 1 is one of the most fluorescent NIR dyes. It has maximum absorption around ~650 nm with extinction coefficient larger than 100,000 M-1 cm-1. It has fluorescence emission around 670 nm, which is pH-independent. Although Oxazine 1 is quite stable from pH 4 to pH 10, it might be reduced by some biological molecules (such as DTT, GSH etc) at high concentration. Oxazine 1 is also an efficient laser dye tunable around 670 nm.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Oxazine 1 *CAS 24796-94-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 235.91 µL | 1.18 mL | 2.359 mL | 11.796 mL | 23.591 mL |
| 5 mM | 47.182 µL | 235.91 µL | 471.821 µL | 2.359 mL | 4.718 mL |
| 10 mM | 23.591 µL | 117.955 µL | 235.91 µL | 1.18 mL | 2.359 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
| DiTO™-1 [equivalent to TOTO®-1] *5 mM DMSO Solution* *CAS 143413-84-7* | 514 | 531 | 1170001 |
| DiYO™-1 [equivalent to YOYO®-1] *5 mM DMSO Solution* | 491 | 508 | 989001 |
| JC-1 [5,5,6,6-Tetrachloro-1,1,3,3-tetraethylbenzimidazolylcarbocyanine iodide] *CAS#: 3520-43-2* | 515 | 530 | 1950001 |
| BTA-1 [2-(4'-(methylamino)phenyl)benzothiazole] | 349 | 421 | - |
| DiYO™-1 [equivalent to YOYO®-1] | 491 | 508 | 989001 |
| DiPO™-1 [equivalent to POPO®-1] *1 mM DMSO Solution* | 433 | 457 | 920001 |
| EthD-1 [Ethidium Homodimer-1] | 528 | 617 | - |
Citations
View all 3 citations: Citation Explorer
Monitoring Methionine Decarboxylase by a Supramolecular Tandem Assay
Authors: Zheng, Zhe and Ren, Siying and Geng, Wen-Chao and Cui, Xuexian and Wu, Bian and Wang, Hong
Journal: Chemistry--An Asian Journal (2022): e202200106
Authors: Zheng, Zhe and Ren, Siying and Geng, Wen-Chao and Cui, Xuexian and Wu, Bian and Wang, Hong
Journal: Chemistry--An Asian Journal (2022): e202200106
A general method to improve fluorophores for live-cell and single-molecule microscopy
Authors: Grimm, Jonathan B and English, Brian P and Chen, Jiji and Slaughter, Joel P and Zhang, Zhengjian and Revyakin, Andrey and Patel, Ronak and Macklin, John J and Normanno, Davide and Singer, Robert H and others, undefined
Journal: Nature methods (2015): 244--250
Authors: Grimm, Jonathan B and English, Brian P and Chen, Jiji and Slaughter, Joel P and Zhang, Zhengjian and Revyakin, Andrey and Patel, Ronak and Macklin, John J and Normanno, Davide and Singer, Robert H and others, undefined
Journal: Nature methods (2015): 244--250
References
View all 11 references: Citation Explorer
Controlling the fluorescence of ordinary oxazine dyes for single-molecule switching and superresolution microscopy
Authors: Vogelsang J, Cordes T, Forthmann C, Steinhauer C, Tinnefeld P.
Journal: Proc Natl Acad Sci U S A (2009): 8107
Authors: Vogelsang J, Cordes T, Forthmann C, Steinhauer C, Tinnefeld P.
Journal: Proc Natl Acad Sci U S A (2009): 8107
Analysis of wave packet motion in frequency and time domain: oxazine 1
Authors: Braun M, Sobotta C, Durr R, Pulvermacher H, Malkmus S.
Journal: J Phys Chem A (2006): 9793
Authors: Braun M, Sobotta C, Durr R, Pulvermacher H, Malkmus S.
Journal: J Phys Chem A (2006): 9793
Effects of pH and electrolyte concentration on the binding between a humic acid and an oxazine dye
Authors: Zanini GP, Avena MJ, Fiol S, Arce F.
Journal: Chemosphere (2006): 430
Authors: Zanini GP, Avena MJ, Fiol S, Arce F.
Journal: Chemosphere (2006): 430
Chirp dependence of wave packet motion in oxazine 1
Authors: Malkmus S, Durr R, Sobotta C, Pulvermacher H, Zinth W, Braun M.
Journal: J Phys Chem A (2005): 10488
Authors: Malkmus S, Durr R, Sobotta C, Pulvermacher H, Zinth W, Braun M.
Journal: J Phys Chem A (2005): 10488
Oxazine 170 induces DNA:RNA:DNA triplex formation
Authors: Song G, Xing F, Qu X, Chaires JB, Ren J.
Journal: J Med Chem (2005): 3471
Authors: Song G, Xing F, Qu X, Chaires JB, Ren J.
Journal: J Med Chem (2005): 3471
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