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OG514 succinimidyl ester [equivalent to Oregon Green® 514 succinimidyl ester]

OG514 is the same fluorophore as Oregon Green 514 (Oregon Green® is the trademark of ThermoFisher). OG514 Succinimidyl Ester is an amine-reactive compound used for creating green, fluorescent bioconjugates. It has excitation/emission maxima approximately 489/526 nm. It is an excellent replacement for rhodamine 6G that has poor solubility in most common solvents, thus practically hard to use. Although it is a fluorescein derivative, it has significantly improved photostability and a lower pKa, making its fluorescence essentially pH insensitive in the physiological pH range. OG514 Succinimidyl Ester can be conveniently used to label antibodies, peptides, amino-modified oligos.

Spectrum

References

View all 5 references: Citation Explorer
A study on the safety and therapeutic effect of Xiaoyaosan on depressive disorder related dry eye disease in a murine animal model.
Authors: Li, Jie and Yan, Ke and Liu, Zhaolin and Lin, Xiang and Huang, Yu and Shi, Jian and Li, Dongdong and Yao, Xiaolei and Liu, Zuguo and Peng, Qinghua
Journal: Journal of histotechnology (2022): 161-171
Cell quiescence correlates with enhanced glioblastoma cell invasion and cytotoxic resistance.
Authors: Atkins, Ryan J and Stylli, Stanley S and Kurganovs, Natalie and Mangiola, Stefano and Nowell, Cameron J and Ware, Thomas M and Corcoran, Niall M and Brown, Daniel V and Kaye, Andrew H and Morokoff, Andrew and Luwor, Rodney B and Hovens, Christopher M and Mantamadiotis, Theo
Journal: Experimental cell research (2019): 353-364
Fluorescent dye incorporation causes weakened gene association and intracellular aggregate formation in nonviral carriers.
Authors: Foster, Abbygail A and Ross, Nikki L and Sullivan, Millicent O
Journal: The journal of gene medicine (2015): 69-79
A folate receptor-α-specific ligand that targets cancer tissue and not sites of inflammation.
Authors: Vaitilingam, Balasubramanian and Chelvam, Venkatesh and Kularatne, Sumith A and Poh, Scott and Ayala-Lopez, Wilfredo and Low, Philip S
Journal: Journal of nuclear medicine : official publication, Society of Nuclear Medicine (2012): 1127-34
Monolayer-functionalized microfluidics devices for optical sensing of acidity.
Authors: Mela, P and Onclin, S and Goedbloed, M H and Levi, S and Garcia-Parajo, M F and van Hulst, N F and Ravoo, B J and Reinhoudt, D N and van den Berg, A
Journal: Lab on a chip (2005): 163-70
Page updated on November 21, 2024

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Catalog Number717
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Physical properties

Molecular weight

609.43

Solvent

DMSO

Spectral properties

Correction Factor (280 nm)

0.19

Extinction coefficient (cm -1 M -1)

85000

Excitation (nm)

513

Emission (nm)

533

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
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