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OG488 maleimide [equivalent to Oregon Green® 488 maleimide]
OG488 maleimide is the same molecule to Oregon Green® 488 maleimide (Invitrogen #O6034, Oregon Green® is the Trademark of Invitrogen). This fluorinated analog of fluorescein has higher photostability and a lower pKa ~ 4.7 while fluorescein maleimide has pKa ~6.4. The decreased pKa of OG488 maleimide makes its fluorescence essentially pH insensitive in the physiological pH range. This thiol-reactive OG488 maleimide can be used to make bright green fluorescent bioconjugates with excitation/emission maxima ~496/524 nm. It is an excellent replacement to fluorescein maleimide that has severe pH dependence and low photostability.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of OG488 maleimide [equivalent to Oregon Green® 488 maleimide] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 215.82 µL | 1.079 mL | 2.158 mL | 10.791 mL | 21.582 mL |
| 5 mM | 43.164 µL | 215.82 µL | 431.639 µL | 2.158 mL | 4.316 mL |
| 10 mM | 21.582 µL | 107.91 µL | 215.82 µL | 1.079 mL | 2.158 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Citations
View all 1 citations: Citation Explorer
Development of cell-penetration PG-surfactants and its application in external peptide delivery to cytosol
Authors: Sumito, Natsumi and Koeda, Shuhei and Umezawa, Naoki and Inoue, Yasumichi and Tsukiji, Shinya and Higuchi, Tsunehiko and Mizuno, Toshihisa
Journal: Bioconjugate Chemistry (2020): 821--833
Authors: Sumito, Natsumi and Koeda, Shuhei and Umezawa, Naoki and Inoue, Yasumichi and Tsukiji, Shinya and Higuchi, Tsunehiko and Mizuno, Toshihisa
Journal: Bioconjugate Chemistry (2020): 821--833
References
View all 14 references: Citation Explorer
Biodegradable polymersomes as carriers and release systems for paclitaxel using Oregon Green(R) 488 labeled paclitaxel as a model compound
Authors: Lee JS, Feijen J.
Journal: J Control Release (2012): 312
Authors: Lee JS, Feijen J.
Journal: J Control Release (2012): 312
Microscopic imaging of intracellular calcium in live cells using lifetime-based ratiometric measurements of Oregon Green BAPTA-1
Authors: Lattarulo C, Thyssen D, Kuchibholta KV, Hyman BT, Bacskaiq BJ.
Journal: Methods Mol Biol (2011): 377
Authors: Lattarulo C, Thyssen D, Kuchibholta KV, Hyman BT, Bacskaiq BJ.
Journal: Methods Mol Biol (2011): 377
Pharmacokinetics of intraocular drug delivery of Oregon green 488-labeled triamcinolone by subtenon injection using ocular fluorophotometry in rabbit eyes
Authors: Lee SJ, Kim ES, Geroski DH, McCarey BE, Edelhauser HF.
Journal: Invest Ophthalmol Vis Sci (2008): 4506
Authors: Lee SJ, Kim ES, Geroski DH, McCarey BE, Edelhauser HF.
Journal: Invest Ophthalmol Vis Sci (2008): 4506
Trans-scleral permeability of Oregon green 488
Authors: Lee SJ, Kim SJ, Kim ES, Geroski DH, McCarey BE, Edelhauser HF.
Journal: J Ocul Pharmacol Ther (2008): 579
Authors: Lee SJ, Kim SJ, Kim ES, Geroski DH, McCarey BE, Edelhauser HF.
Journal: J Ocul Pharmacol Ther (2008): 579
The Pennsylvania Green Fluorophore: a hybrid of Oregon Green and Tokyo Green for the construction of hydrophobic and pH-insensitive molecular probes
Authors: Mottram LF, Boonyarattanakalin S, Kovel RE, Peterson BR.
Journal: Org Lett (2006): 581
Authors: Mottram LF, Boonyarattanakalin S, Kovel RE, Peterson BR.
Journal: Org Lett (2006): 581
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