Calculator
OG488 maleimide [equivalent to Oregon Green® 488 maleimide]
OG488 maleimide is the same molecule to Oregon Green® 488 maleimide (Invitrogen #O6034, Oregon Green® is the Trademark of Invitrogen). This fluorinated analog of fluorescein has higher photostability and a lower pKa ~ 4.7 while fluorescein maleimide has pKa ~6.4. The decreased pKa of OG488 maleimide makes its fluorescence essentially pH insensitive in the physiological pH range. This thiol-reactive OG488 maleimide can be used to make bright green fluorescent bioconjugates with excitation/emission maxima ~496/524 nm. It is an excellent replacement to fluorescein maleimide that has severe pH dependence and low photostability.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of OG488 maleimide [equivalent to Oregon Green® 488 maleimide] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 215.82 µL | 1.079 mL | 2.158 mL | 10.791 mL | 21.582 mL |
| 5 mM | 43.164 µL | 215.82 µL | 431.639 µL | 2.158 mL | 4.316 mL |
| 10 mM | 21.582 µL | 107.91 µL | 215.82 µL | 1.079 mL | 2.158 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Citations
View all 1 citations: Citation Explorer
Development of cell-penetration PG-surfactants and its application in external peptide delivery to cytosol
Authors: Sumito, Natsumi and Koeda, Shuhei and Umezawa, Naoki and Inoue, Yasumichi and Tsukiji, Shinya and Higuchi, Tsunehiko and Mizuno, Toshihisa
Journal: Bioconjugate Chemistry (2020): 821--833
Authors: Sumito, Natsumi and Koeda, Shuhei and Umezawa, Naoki and Inoue, Yasumichi and Tsukiji, Shinya and Higuchi, Tsunehiko and Mizuno, Toshihisa
Journal: Bioconjugate Chemistry (2020): 821--833
References
View all 14 references: Citation Explorer
Biodegradable polymersomes as carriers and release systems for paclitaxel using Oregon Green(R) 488 labeled paclitaxel as a model compound
Authors: Lee JS, Feijen J.
Journal: J Control Release (2012): 312
Authors: Lee JS, Feijen J.
Journal: J Control Release (2012): 312
Microscopic imaging of intracellular calcium in live cells using lifetime-based ratiometric measurements of Oregon Green BAPTA-1
Authors: Lattarulo C, Thyssen D, Kuchibholta KV, Hyman BT, Bacskaiq BJ.
Journal: Methods Mol Biol (2011): 377
Authors: Lattarulo C, Thyssen D, Kuchibholta KV, Hyman BT, Bacskaiq BJ.
Journal: Methods Mol Biol (2011): 377
Pharmacokinetics of intraocular drug delivery of Oregon green 488-labeled triamcinolone by subtenon injection using ocular fluorophotometry in rabbit eyes
Authors: Lee SJ, Kim ES, Geroski DH, McCarey BE, Edelhauser HF.
Journal: Invest Ophthalmol Vis Sci (2008): 4506
Authors: Lee SJ, Kim ES, Geroski DH, McCarey BE, Edelhauser HF.
Journal: Invest Ophthalmol Vis Sci (2008): 4506
Trans-scleral permeability of Oregon green 488
Authors: Lee SJ, Kim SJ, Kim ES, Geroski DH, McCarey BE, Edelhauser HF.
Journal: J Ocul Pharmacol Ther (2008): 579
Authors: Lee SJ, Kim SJ, Kim ES, Geroski DH, McCarey BE, Edelhauser HF.
Journal: J Ocul Pharmacol Ther (2008): 579
The Pennsylvania Green Fluorophore: a hybrid of Oregon Green and Tokyo Green for the construction of hydrophobic and pH-insensitive molecular probes
Authors: Mottram LF, Boonyarattanakalin S, Kovel RE, Peterson BR.
Journal: Org Lett (2006): 581
Authors: Mottram LF, Boonyarattanakalin S, Kovel RE, Peterson BR.
Journal: Org Lett (2006): 581
Page updated on October 28, 2024
Safety data sheet