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Nile Red *CAS 7385-67-3*
Nile red (also known as Nile blue oxazone) is a lipophilic stain. It has environment-sensitive fluorescence. Nile red is intensely fluorescent in a lipid-rich environment while it has minimal fluorescence in aqueous media. It is an excellent vital stain for the detection of intracellular lipid droplets by fluorescence microscopy and flow cytofluorometry. Nile red stains intracellular lipid droplets red. Better selectivity for cytoplasmic lipid droplets can be obtained when the cells are viewed for yellow-gold fluorescence (450-500 nm excitation; >528 nm emission) rather than red fluorescence (515-560 nm excitation; >590 nm emission). It is strongly fluorescent, but only in a hydrophobic environment.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Nile Red *CAS 7385-67-3* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 314.1 µL | 1.57 mL | 3.141 mL | 15.705 mL | 31.41 mL |
| 5 mM | 62.82 µL | 314.1 µL | 628.2 µL | 3.141 mL | 6.282 mL |
| 10 mM | 31.41 µL | 157.05 µL | 314.1 µL | 1.57 mL | 3.141 mL |
Molarity calculator
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Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) |
| Amplite® Red | 571 | 584 |
| LysoBrite™ Red | 576 | 596 |
| Amylite™ Red | 485 | 671 |
Citations
View all 12 citations: Citation Explorer
Characterizing $\alpha$-Synuclein Polymorphs Through AFM, SAA, Spectrophotometry and Digestion Assay
Authors: Chen, W
Journal: (2024)
Authors: Chen, W
Journal: (2024)
SPG15 protein deficits are at the crossroads between lysosomal abnormalities, altered lipid metabolism and synaptic dysfunction
Authors: Marrone, Lara and Marchi, Paolo M and Webster, Christopher P and Marroccella, Raffaele and Coldicott, Ian and Reynolds, Steven and Alves-Cruzeiro, Jo{\~a}o and Yang, Zih-Liang and Higginbottom, Adrian and Khundadze, Mukhran and others,
Journal: Human Molecular Genetics (2022): 2693--2710
Authors: Marrone, Lara and Marchi, Paolo M and Webster, Christopher P and Marroccella, Raffaele and Coldicott, Ian and Reynolds, Steven and Alves-Cruzeiro, Jo{\~a}o and Yang, Zih-Liang and Higginbottom, Adrian and Khundadze, Mukhran and others,
Journal: Human Molecular Genetics (2022): 2693--2710
Dairy Cattle Nutrition and its Effect on Production, Milk, and Human Health
Authors: Swanson, Katherine L
Journal: (2018)
Authors: Swanson, Katherine L
Journal: (2018)
CRISPR/Cas9--mediated Tspo gene mutations lead to reduced mitochondrial membrane potential and steroid formation in MA-10 mouse tumor Leydig cells
Authors: Fan, Jinjiang and Wang, Kevin and Zirkin, Barry and Papadopoulos, Vassilios
Journal: Endocrinology (2018): 1130--1146
Authors: Fan, Jinjiang and Wang, Kevin and Zirkin, Barry and Papadopoulos, Vassilios
Journal: Endocrinology (2018): 1130--1146
Depot-specific characteristics of adipose tissue-derived stromal cells in thyroid-associated orbitopathy
Authors: Wong, Janice Siu Chong and Chu, Wai Kit and Li, Benjamin Fuk-Loi and Pang, Chi-Pui and Chong, Kelvin Kam-lung
Journal: British Journal of Ophthalmology (2018): bjophthalmol--2017
Authors: Wong, Janice Siu Chong and Chu, Wai Kit and Li, Benjamin Fuk-Loi and Pang, Chi-Pui and Chong, Kelvin Kam-lung
Journal: British Journal of Ophthalmology (2018): bjophthalmol--2017
References
View all 73 references: Citation Explorer
A clinical flow cytometric biomarker strategy: validation of peripheral leukocyte phospholipidosis using Nile red
Authors: Halstead BW, Zwickl CM, Morgan RE, Monteith DK, Thomas CE, Bowers RK, Berridge BR.
Journal: J Appl Toxicol (2006): 169
Authors: Halstead BW, Zwickl CM, Morgan RE, Monteith DK, Thomas CE, Bowers RK, Berridge BR.
Journal: J Appl Toxicol (2006): 169
Nile Red-adsorbed gold nanoparticle matrixes for determining aminothiols through surface-assisted laser desorption/ionization mass spectrometry
Authors: Huang YF, Chang HT.
Journal: Anal Chem (2006): 1485
Authors: Huang YF, Chang HT.
Journal: Anal Chem (2006): 1485
West Nile virus epizootiology, central Red River Valley, North Dakota and Minnesota, 2002-2005
Authors: Bell JA, Brewer CM, Mickelson NJ, Garman GW, Vaughan JA.
Journal: Emerg Infect Dis (2006): 1245
Authors: Bell JA, Brewer CM, Mickelson NJ, Garman GW, Vaughan JA.
Journal: Emerg Infect Dis (2006): 1245
Spectroscopic characterization of poly(amidoamine) dendrimers as selective uptake devices: Phenol blue versus Nile red
Authors: Morgan EJ, Rippey JM, Tucker SA.
Journal: Appl Spectrosc (2006): 551
Authors: Morgan EJ, Rippey JM, Tucker SA.
Journal: Appl Spectrosc (2006): 551
Cationic liposomes in mixed didodecyldimethylammonium bromide and dioctadecyldimethylammonium bromide aqueous dispersions studied by differential scanning calorimetry, nile red fluorescence, and turbidity
Authors: Feitosa E, Alves FR, Niemiec A, Real Oliveira ME, Castanheira EM, Baptista AL.
Journal: Langmuir (2006): 3579
Authors: Feitosa E, Alves FR, Niemiec A, Real Oliveira ME, Castanheira EM, Baptista AL.
Journal: Langmuir (2006): 3579
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