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Nile Blue A chloride

Nile blue A is a stain used in biology and histology. It may be used with live or fixed cells and imparts a blue color to cell nuclei. It is used in a variety of commercial DNA color staining formulations for DNA electrophoresis. As it does not require UV trans-illumination for visualization in an agarose gel as with ethidium bromide and other fluorescent stains such SYBR Green or Gel Red, it can be used to observe DNA as it is separated and also as a dye to aid in gel-extraction of DNA fragments without incurring damage by UV-irradiation. The derivatives of Nile Blue A are potential photosensitizers in photodynamic therapy of malignant tumors. These dyes aggregate in the tumor cells, especially in the lipid membranes, and/or are sequestered and concentrated in subcellular organelles.

Spectrum

References

View all 50 references: Citation Explorer
In situ identification of polyhydroxyalkanoate (PHA)-accumulating microorganisms in mixed microbial cultures under feast/famine conditions.
Authors: Sruamsiri, Donhatai and Thayanukul, Parinda and Suwannasilp, Benjaporn Boonchayaanant
Journal: Scientific reports (2020): 3752
Ratiometric Strategy for Electrochemical Sensing of Carbaryl Residue in Water and Vegetable Samples.
Authors: Zhang, Min and Zhang, Zeyuan and Yang, Yanjing and Zhang, Yi and Wang, Yufei and Chen, Xinyi
Journal: Sensors (Basel, Switzerland) (2020)
A SERS aptasensor for simultaneous multiple pathogens detection using gold decorated PDMS substrate.
Authors: Duan, Nuo and Shen, Mofei and Qi, Shuo and Wang, Wenyue and Wu, Shijia and Wang, Zhouping
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2020): 118103
Development of novel optical pH sensors based on coumarin 6 and nile blue A encapsulated in resin particles and specific support materials.
Authors: Duong, Hong Dinh and Shin, Younsook and Rhee, Jong Il
Journal: Materials science & engineering. C, Materials for biological applications (2020): 110323
SERS-Based Immunoassays for the Detection of Botulinum Toxins A and B Using Magnetic Beads.
Authors: Kim, Kihyun and Choi, Namhyun and Jeon, Jun Ho and Rhie, Gi-Eun and Choo, Jaebum
Journal: Sensors (Basel, Switzerland) (2019)
Page updated on December 17, 2024

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Catalog Number59
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Physical properties

Molecular weight

353.85

Solvent

DMSO

Spectral properties

Absorbance (nm)

631

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501

CAS

2381-85-3
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