Newport Green™ DCF Diacetate *Cell Permeant*

• Live-cell compatible – Membrane-permeant diacetate form enables passive diffusion and intracellular retention after esterase activation.
• Zinc-selective – Moderate Zn²⁺ affinity (K_d ≈ 1 μM) with minimal cross-reactivity to Ca²⁺ and other divalent cations.
• Sensitive to zinc flux – Ideal for detecting rapid Zn²⁺ transients in neurons and other excitable cells.
• FITC-compatible – Excitation/emission (~505/535 nm) suited for standard FITC filter sets across common fluorescence platforms.
• Stable intracellular signal – Water-soluble, de-esterified dye is retained in the cytoplasm with minimal leakage.

Newport Green™ DCF Diacetate is a cell-permeable zinc indicator optimized for the quantitative detection of intracellular Zn²⁺ in live-cell systems. In its diacetate-protected form, the probe passively diffuses across the plasma membrane. Upon cellular entry, endogenous cytosolic esterases hydrolyze the acetyl groups, generating the de-esterified Newport Green™ DCF dye, which is water-soluble and effectively retained within the cytoplasm. The activated dye exhibits a moderate affinity for Zn²⁺ (K_d ≈ 1 μM), enabling sensitive detection of physiologically relevant concentrations of labile zinc. Importantly, it displays minimal affinity for Ca²⁺ (K_d > 100 μM), providing high selectivity for Zn²⁺ over other intracellular divalent cations. This selectivity makes it particularly suitable for investigating dynamic Zn²⁺ signaling events in excitable cells, such as neurons, where zinc influx is mediated by glutamatergic stimulation or voltage-gated ion channels. Newport Green™ DCF exhibits excitation and emission maxima at approximately 505 nm and 535 nm, respectively, ensuring compatibility with standard FITC filter sets for applications including fluorescence microscopy, flow cytometry, and high-content imaging.