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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 511.50 |
Solvent | Water |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
MycoLight™ PMA [Propidium Monoazide] *5 mM Water Solution* |
Related products
Overview | ![]() ![]() |
CAS 91416-20-5 | Molecular weight 511.50 |
The common PCR assays have been routinely used for simple, sensitive, and specific pathogen identification. However, regular PCR assays cannot be used to distinguish between live and dead cells. The number of pathogens is always overestimated by the regular PCR assays. Viability PCR (vPCR) is an evolution of PCR. The simple pre-treatment of a test sample by specifically intercalating a photo-reactive reagent to the DNA of dead cells enables the selective PCR detection of DNA in live cells. The capability to selectively detect the DNA of living cells becomes very important, such as, in the fields of food safety, water quality control, infectious diseases, veterinary applications and ecological dynamics etc. Propidium monoazide (PMA) is one of the most common vPCR reagents for the selective detection of DNA of live cells. The PMA is a membrane-impermeable dye that selectively penetrates membranes of dead cells. Once inside a dead cell, PMA intercalates into the DNA and can be covalently cross-linked to it. This effect will strongly inhibit PCR amplification and leads to elimination of positive signals from dead cells. The PMA-based vPCR has been used for detecting emetic and non-emetic B. cereus and other bacterial pathogens. However, PMA is extremely light-sensitive, thus needs to be operated under the dark conditions. AAT Bioquest recently introduced MycoLight™ vPCR350. It is much less light sensitive (to the room light) than PMA, thus can be used under room light. It is a significantly improved version of PMA-like vPCR reagent. It is a novel non-fluorescent DNA modifier specifically designed for viability PCR (vPCR) targeting microorganisms such as bacteria, eukaryotes, viruses, and fungi. It represents a significant advancement over the widely used viability dye PMA (propidium monoazide), offering enhanced performance and specificity compared to PMA.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of MycoLight™ PMA [Propidium Monoazide] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 195.503 µL | 977.517 µL | 1.955 mL | 9.775 mL | 19.55 mL |
5 mM | 39.101 µL | 195.503 µL | 391.007 µL | 1.955 mL | 3.91 mL |
10 mM | 19.55 µL | 97.752 µL | 195.503 µL | 977.517 µL | 1.955 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Images
Figure 1. Propidium monoazide (PMA) is one of the most common vPCR reagents for the selective detection of DNA of live cells. The PMA is a membrane-impermeable dye that selectively penetrates membranes of dead cells. Once inside a dead cell, PMA intercalates into the DNA and can be covalently cross-linked to it.
References
View all 12 references: Citation Explorer
Optimization and Testing of a Commercial Viability PCR Protocol to Detect Escherichia coli in Whole Blood.
Authors: Jones, Kristi L and Cunha, Federico and Casaro, Segundo and Galvão, Klibs N
Journal: Microorganisms (2024)
Authors: Jones, Kristi L and Cunha, Federico and Casaro, Segundo and Galvão, Klibs N
Journal: Microorganisms (2024)
Understanding the reaction balances behind the viability PCR protocols based on photoreactive dyes.
Authors: Codony, F and Barreto, L and Agustí, G and Asensio, D
Journal: Journal of microbiological methods (2023): 106737
Authors: Codony, F and Barreto, L and Agustí, G and Asensio, D
Journal: Journal of microbiological methods (2023): 106737
An Improved Real-Time Viability PCR Assay to Detect Salmonella in a Culture-Independent Era.
Authors: Thilakarathna, Surangi H and Stokowski, Taryn and Chui, Linda
Journal: International journal of molecular sciences (2022)
Authors: Thilakarathna, Surangi H and Stokowski, Taryn and Chui, Linda
Journal: International journal of molecular sciences (2022)
Viability-based quantification of antibiotic resistance genes and human fecal markers in wastewater effluent and receiving waters.
Authors: Eramo, Alessia and Morales Medina, William R and Fahrenfeld, Nicole L
Journal: The Science of the total environment (2019): 495-502
Authors: Eramo, Alessia and Morales Medina, William R and Fahrenfeld, Nicole L
Journal: The Science of the total environment (2019): 495-502
Assessment of bacterial viability: a comprehensive review on recent advances and challenges.
Authors: Kumar, Shravanthi S and Ghosh, Asit Ranjan
Journal: Microbiology (Reading, England) (2019): 593-610
Authors: Kumar, Shravanthi S and Ghosh, Asit Ranjan
Journal: Microbiology (Reading, England) (2019): 593-610
Droplet digital PCR improves absolute quantification of viable lactic acid bacteria in faecal samples.
Authors: Gobert, Guillaume and Cotillard, Aurélie and Fourmestraux, Candice and Pruvost, Laurence and Miguet, Jean and Boyer, Mickaël
Journal: Journal of microbiological methods (2018): 64-73
Authors: Gobert, Guillaume and Cotillard, Aurélie and Fourmestraux, Candice and Pruvost, Laurence and Miguet, Jean and Boyer, Mickaël
Journal: Journal of microbiological methods (2018): 64-73
Molecular Viability Testing of UV-Inactivated Bacteria.
Authors: Weigel, Kris M and Nguyen, Felicia K and Kearney, Moira R and Meschke, John S and Cangelosi, Gerard A
Journal: Applied and environmental microbiology (2017)
Authors: Weigel, Kris M and Nguyen, Felicia K and Kearney, Moira R and Meschke, John S and Cangelosi, Gerard A
Journal: Applied and environmental microbiology (2017)
Viability-PCR Shows That NAAT Detects a High Proportion of DNA from Non-Viable Chlamydia trachomatis.
Authors: Janssen, Kevin J H and Hoebe, Christian J P A and Dukers-Muijrers, Nicole H T M and Eppings, Lisanne and Lucchesi, Mayk and Wolffs, Petra F G
Journal: PloS one (2016): e0165920
Authors: Janssen, Kevin J H and Hoebe, Christian J P A and Dukers-Muijrers, Nicole H T M and Eppings, Lisanne and Lucchesi, Mayk and Wolffs, Petra F G
Journal: PloS one (2016): e0165920
A multicenter study of viable PCR using propidium monoazide to detect Legionella in water samples.
Authors: Scaturro, Maria and Fontana, Stefano and Dell'eva, Italo and Helfer, Fabrizia and Marchio, Michele and Stefanetti, Maria Vittoria and Cavallaro, Mario and Miglietta, Marilena and Montagna, Maria Teresa and De Giglio, Osvalda and Cuna, Teresa and Chetti, Leonarda and Sabattini, Maria Antonietta Bucci and Carlotti, Michela and Viggiani, Mariagabriella and Stenico, Alberta and Romanin, Elisa and Bonanni, Emma and Ottaviano, Claudio and Franzin, Laura and Avanzini, Claudio and Demarie, Valerio and Corbella, Marta and Cambieri, Patrizia and Marone, Piero and Rota, Maria Cristina and Bella, Antonino and Ricci, Maria Luisa
Journal: Diagnostic microbiology and infectious disease (2016): 283-288
Authors: Scaturro, Maria and Fontana, Stefano and Dell'eva, Italo and Helfer, Fabrizia and Marchio, Michele and Stefanetti, Maria Vittoria and Cavallaro, Mario and Miglietta, Marilena and Montagna, Maria Teresa and De Giglio, Osvalda and Cuna, Teresa and Chetti, Leonarda and Sabattini, Maria Antonietta Bucci and Carlotti, Michela and Viggiani, Mariagabriella and Stenico, Alberta and Romanin, Elisa and Bonanni, Emma and Ottaviano, Claudio and Franzin, Laura and Avanzini, Claudio and Demarie, Valerio and Corbella, Marta and Cambieri, Patrizia and Marone, Piero and Rota, Maria Cristina and Bella, Antonino and Ricci, Maria Luisa
Journal: Diagnostic microbiology and infectious disease (2016): 283-288
Recent developments in the use of viability dyes and quantitative PCR in the food microbiology field.
Authors: Elizaquível, P and Aznar, R and Sánchez, G
Journal: Journal of applied microbiology (2014): 1-13
Authors: Elizaquível, P and Aznar, R and Sánchez, G
Journal: Journal of applied microbiology (2014): 1-13