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MUP [4-Methylumbelliferyl phosphate, free acid] *CAS 3368-04-5*
A fluorogenic substrate for phosphatases. It is widely used for detecting phosphatases in solution. However this phosphatase substrate is not well suited for living cell or continuous assays since MU (4-methylumbelliferone), the enzymatic product, which only develops maximum fluorescence at pH value of >10. Thus it is also difficult to use MUP for the detection of phosphatases that have acidic optimal pH range such as acid phosphatases. AAT Bioquest is pleased to offer MUP Plus that is developed to address this pH limitation associated with MUP substrates.
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Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of MUP [4-Methylumbelliferyl phosphate, free acid] *CAS 3368-04-5* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 390.396 µL | 1.952 mL | 3.904 mL | 19.52 mL | 39.04 mL |
| 5 mM | 78.079 µL | 390.396 µL | 780.793 µL | 3.904 mL | 7.808 mL |
| 10 mM | 39.04 µL | 195.198 µL | 390.396 µL | 1.952 mL | 3.904 mL |
Molarity calculator
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References
View all 76 references: Citation Explorer
Application of intracellular alkaline phosphatase activity measurement in detection of neutrophil adherence in vitro
Authors: Bednarska K, Klink M, Sulowska Z.
Journal: Mediators Inflamm (2006): 19307
Authors: Bednarska K, Klink M, Sulowska Z.
Journal: Mediators Inflamm (2006): 19307
Evaluation of an alternative method for the enumeration and confirmation of Clostridium perfringens from treated and untreated sewages
Authors: Wohlsen T, Bayliss J, Gray B, Bates J, Katouli M.
Journal: Lett Appl Microbiol (2006): 438
Authors: Wohlsen T, Bayliss J, Gray B, Bates J, Katouli M.
Journal: Lett Appl Microbiol (2006): 438
Fluorometric cell-ELISA for quantifying rabies infection and heparin inhibition
Authors: Rincon V, Corredor A, Martinez-Gutierrez M, Castellanos JE.
Journal: J Virol Methods (2005): 33
Authors: Rincon V, Corredor A, Martinez-Gutierrez M, Castellanos JE.
Journal: J Virol Methods (2005): 33
Relative movement and soil fixation of soluble organic and inorganic phosphorus
Authors: Anderson BH, Magdoff FR.
Journal: J Environ Qual (2005): 2228
Authors: Anderson BH, Magdoff FR.
Journal: J Environ Qual (2005): 2228
Purification and partial characterization of an acid phosphatase from Spirodela oligorrhiza and its affinity for selected organophosphate pesticides
Authors: Hoehamer CF, Mazur CS, Wolfe NL.
Journal: J Agric Food Chem (2005): 90
Authors: Hoehamer CF, Mazur CS, Wolfe NL.
Journal: J Agric Food Chem (2005): 90
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