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MUGlcU [4-Methylumbelliferyl-beta-D-glucuronide] *CAS 881005-91-0*
MUGlcU is a fluorogenic MU substrate for detecting glucuronidase in cell extracts and purified enzyme preparations using a fluorescence microplate reader or fluorometer. The organic and inorganic esters and glycosides of 7-hydroxy-4-methylcoumarin (4-methylumbelliferone, MU) are among the most commonly used fluorogenic substrates. These substrates yield water-soluble blue fluorescent coumarin fluorophores. MU develops maximum fluorescence above pH 10.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of MUGlcU [4-Methylumbelliferyl-beta-D-glucuronide] *CAS 881005-91-0* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 283.857 µL | 1.419 mL | 2.839 mL | 14.193 mL | 28.386 mL |
| 5 mM | 56.771 µL | 283.857 µL | 567.714 µL | 2.839 mL | 5.677 mL |
| 10 mM | 28.386 µL | 141.929 µL | 283.857 µL | 1.419 mL | 2.839 mL |
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References
View all 4 references: Citation Explorer
Coumarin Schiff-bases: as antioxidant and possibly anti-inflammatory agents
Authors: Gacche RN, Gond DS, Dhole NA, Dawane BS.
Journal: J Enzyme Inhib Med Chem (2006): 157
Authors: Gacche RN, Gond DS, Dhole NA, Dawane BS.
Journal: J Enzyme Inhib Med Chem (2006): 157
Examination of lectins, polysaccharopeptide, polysaccharide, alkaloid, coumarin and trypsin inhibitors for inhibitory activity against human immunodeficiency virus reverse transcriptase and glycohydrolases
Authors: Wang HX, Ng TB.
Journal: Planta Med (2001): 669
Authors: Wang HX, Ng TB.
Journal: Planta Med (2001): 669
Induction of indoleamine 2,3-dioxygenase in alveolar interstitial cells of mouse lung by bacterial lipopolysaccharide
Authors: Urade Y, Yoshida R, Kitamura H, Hayaishi O.
Journal: J Biol Chem (1983): 6621
Authors: Urade Y, Yoshida R, Kitamura H, Hayaishi O.
Journal: J Biol Chem (1983): 6621
Determination of dicumarol metabolites in bile of rats
Authors: Conway WD, Lee FH, Neufeld L.
Journal: J Pharm Sci (1975): 1158
Authors: Conway WD, Lee FH, Neufeld L.
Journal: J Pharm Sci (1975): 1158
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