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MUG [4-Methylumbelliferyl-beta-D-galactopyranoside] *CAS 6160-78-7*
4-Methylumbelliferyl-beta-D-galactopyranoside (MUG) is a fluorogenic MU substrate for detecting galactosidase in cell extracts and purified enzyme preparations using a fluorescence microplate reader or fluorometer. The organic and inorganic esters and glycosides of 7-hydroxy-4-methylcoumarin (4-methylumbelliferone, MU) are among the most commonly used fluorogenic substrates. These substrates yield water-soluble blue fluorescent coumarin fluorophores. MU develops maximum fluorescence above pH 10.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of MUG [4-Methylumbelliferyl-beta-D-galactopyranoside] *CAS 6160-78-7* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 295.578 µL | 1.478 mL | 2.956 mL | 14.779 mL | 29.558 mL |
| 5 mM | 59.116 µL | 295.578 µL | 591.156 µL | 2.956 mL | 5.912 mL |
| 10 mM | 29.558 µL | 147.789 µL | 295.578 µL | 1.478 mL | 2.956 mL |
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References
View all 18 references: Citation Explorer
Intraperitoneal linear polyethylenimine (L-PEI)-mediated gene delivery to ovarian carcinoma nodes in mice
Authors: Louis MH, Dutoit S, Denoux Y, Erbacher P, Desl and es E, Behr JP, Gauduchon P, Poulain L.
Journal: Cancer Gene Ther (2006): 367
Authors: Louis MH, Dutoit S, Denoux Y, Erbacher P, Desl and es E, Behr JP, Gauduchon P, Poulain L.
Journal: Cancer Gene Ther (2006): 367
Characterization of an unusual cold-active beta-glucosidase belonging to family 3 of the glycoside hydrolases from the psychrophilic isolate Paenibacillus sp. strain C7
Authors: Shipkowski S, Brenchley JE.
Journal: Appl Environ Microbiol (2005): 4225
Authors: Shipkowski S, Brenchley JE.
Journal: Appl Environ Microbiol (2005): 4225
Catheter-based antegrade intracoronary viral gene delivery with coronary venous blockade
Authors: Hayase M, Del Monte F, Kawase Y, Macneill BD, McGregor J, Yoneyama R, Hoshino K, Tsuji T, De Gr and AM, Gwathmey JK, Frangioni JV, Hajjar RJ.
Journal: Am J Physiol Heart Circ Physiol (2005): H2995
Authors: Hayase M, Del Monte F, Kawase Y, Macneill BD, McGregor J, Yoneyama R, Hoshino K, Tsuji T, De Gr and AM, Gwathmey JK, Frangioni JV, Hajjar RJ.
Journal: Am J Physiol Heart Circ Physiol (2005): H2995
Neuronal Nitric Oxide Synthase Activity in the Paraventricular Nucleus Buffers Central Endothelin-1- induced Pressor Response and Vasopressin Secretion
Authors: Rossi NF, Black SM, Telemaque-Potts S, Chen H.
Journal: J Cardiovasc Pharmacol (2004): S283
Authors: Rossi NF, Black SM, Telemaque-Potts S, Chen H.
Journal: J Cardiovasc Pharmacol (2004): S283
A novel synthetic peptide derivative from lactoferrin exhibiting antimicrobial activity
Authors: Ramesh CV, Kamini NR, Puvanakrishnan R.
Journal: Biotechnol Appl Biochem (2004): 271
Authors: Ramesh CV, Kamini NR, Puvanakrishnan R.
Journal: Biotechnol Appl Biochem (2004): 271
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