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AAT Bioquest

MP650 Dye qPCR Calibration Solution *10,000X*

MP650 is designed to have identical spectra to those of Mustang Purple. Real-time thermal cyclers require calibration to generate a spectral profile for the fluorescence signal. Since instruments can differ in fluorescence detection sensitivity, the concentrations needed to calibrate any dye, such as MP650, will depend on the device used. The AAT Bioquest MP650 dye qPCR Calibration Solution supplied as a 10,000X concentrate is compatible with a wide variety of qPCR instruments, including the ABI7500 Fast, QuantStudio™, and ViiA™ 7 systems. Calibration and verification should be run every six months or as needed. Please refer to the instrument maintenance guide for complete instructions on use.

Example protocol

SAMPLE EXPERIMENTAL PROTOCOL

This generic protocol is suitable for the ABI7500 FAST qPCR instrument. For complete instructions on use, refer to the maintenance guide for your instrument.

Prepare a MP650 calibration plate:
  1. Prepare an MP650 Dye qPCR Calibration working solution by mixing 1 µL of the 10,000X MP650 Dye qPCR Calibration Solution with 10 mL of nuclease-free water or qPCR buffer.

    Note: The final dye concentration must be optimized based on the calibration requirements for your instrument. Refer to the manufacturer’s maintenance guide for detailed instructions.

  2. Add 20 µL of the MP650 Dye qPCR Calibration working solution to all wells of a reaction plate.

    Note: The volume may vary depending on your instrument. Refer to the manufacturer’s maintenance guide for detailed instructions.

  3. Seal the plate with an optical adhesive cover.

  4. Centrifuge the plate for 2 minutes at < 1500 rpm.

  5. Check that the liquid in each well of the plate is at the bottom of the well. If not, centrifuge the plate again at a slightly higher rpm and for a longer period of time.

  6. For complete calibration instructions, refer to the maintenance guide for your instrument.

Spectrum

References

View all 24 references: Citation Explorer
Detection of Sulfoquinovosidase Activity in Cell Lysates Using Activity-Based Probes.
Authors: Li, Zirui and Pickles, Isabelle and Sharma, Mahima and Melling, Benjamin and Codee, Jeroen and Pallasdies, Luise and Williams, Spencer and Overkleeft, Herman and Davies, Gideon John
Journal: Angewandte Chemie (International ed. in English) (2024): e202401358
Toxicity Studies of Cardiac-Targeting Peptide Reveal a Robust Safety Profile.
Authors: Sahagun, Daniella A and Lopuszynski, Jack B and Feldman, Kyle S and Pogodzinski, Nicholas and Zahid, Maliha
Journal: Pharmaceutics (2024)
Analytical sensitivity and clinical performance of "COVID-19 RT-PCR Real TM FAST (CY5) (ATGen, Uruguay) and "ECUGEN SARS-CoV-2 RT-qPCR" (UDLA-STARNEWCORP, Ecuador)": High quality-low cost local SARS-CoV-2 tests for South America.
Authors: Freire-Paspuel, Byron and Morales-Jadan, Diana and Zambrano-Mila, Marlon and Perez, Franklin and Garcia-Bereguiain, Miguel Angel
Journal: PLoS neglected tropical diseases (2022): e0010082
Sequence-dependence of Cy3 and Cy5 dyes in 3' terminally-labeled single-stranded DNA.
Authors: Kekić, Tadija and Lietard, Jory
Journal: Scientific reports (2022): 14803
Multiplex qPCR for differentiation of Mycobacterium avium subspecies paratuberculosis in active and passive infection of goats.
Authors: Pachoori, Anjali and Gururaj, K and Sachan, Supriya and Sharma, Deepansh
Journal: Applied microbiology and biotechnology (2022): 4705-4717
Page updated on December 17, 2024

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Catalog Number67039
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Physical properties

Solvent

Water

Spectral properties

Extinction coefficient (cm -1 M -1)

2500001

Excitation (nm)

639

Emission (nm)

653

Quantum yield

0.291

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501

Platform

qPCR

Recommended platePCR Microplate
Instrument specification(s)MP650 or Mustang Purple Filter
ABI 7500 FAST system MP650 dye spectrum.
ABI 7500 FAST system MP650 dye spectrum.
ABI 7500 FAST system MP650 dye spectrum.