mFluor™ Violet 540 Biotin Conjugate
mFluor™ Violet 540 Biotin Conjugate contains mFluor™ Violet 540 fluorophore that is well excited by the common 405 nm violet laser. It has a similar spectrum to Pacific Orange and demonstrates excellent avidin binding and strong orange fluorescence. It has avidin-binding properties similar to native biotin in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers. mFluor™ Violet 540 Biotin Conjugate can be used for detecting avidin, streptavidin or neutravidin conjugates. It is a water-soluble reagent and has pH independent fluorescence from pH 4 to pH 10. The flexible spacer between biotin moiety and fluorescent tag minimizes the steric hindrance involved in binding to avidin, streptavidin or neutravidin.
Spectrum
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Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
mFluor™ Violet 540-streptavidin conjugate | 402 | 535 | 180001 | 0.211 | 1.326 | 0.543 |
mFluor™ Violet 500 Biotin Conjugate | 410 | 501 | 250001 | 0.811 | 0.769 | 0.365 |
References
View all 50 references: Citation Explorer
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Journal: Journal of drug targeting (2022): 1-9
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Journal: Journal of drug targeting (2022): 1-9
Investigation and validation of labelling loop mediated isothermal amplification (LAMP) products with different nucleotide modifications for various downstream analysis.
Authors: Warmt, Christian and Yaslanmaz, Ceren and Henkel, Jörg
Journal: Scientific reports (2022): 7137
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Journal: Scientific reports (2022): 7137
A ratiometric fluorescent nanoprobe for signal amplification monitoring of intracellular telomerase activity.
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Journal: Analytical and bioanalytical chemistry (2022): 1891-1898
Authors: Ma, Dandan and Bai, Huiyun and Li, Junbo and Li, Yintao and Song, Lihua and Zheng, Jinping and Miao, Congxiu
Journal: Analytical and bioanalytical chemistry (2022): 1891-1898
A low-noise ratiometric fluorescence biosensor for detection of Pb2+ based on DNAzyme and exonuclease III-assisted cascade signal amplification.
Authors: Jin, Huali and Liu, Ruike and Bai, Tian and Wei, Min and He, Baoshan and Suo, Zhiguang
Journal: Analytical and bioanalytical chemistry (2022): 1899-1907
Authors: Jin, Huali and Liu, Ruike and Bai, Tian and Wei, Min and He, Baoshan and Suo, Zhiguang
Journal: Analytical and bioanalytical chemistry (2022): 1899-1907
Detection of cholera toxin with surface plasmon field-enhanced fluorescent spectroscopy.
Authors: Seherler, Sebnem and Bozdogan, Anil and Ozal Ildeniz, Tugba Arzu and Kok, Fatma Nese and Anac Sakir, Ilke
Journal: Biotechnology and applied biochemistry (2021)
Authors: Seherler, Sebnem and Bozdogan, Anil and Ozal Ildeniz, Tugba Arzu and Kok, Fatma Nese and Anac Sakir, Ilke
Journal: Biotechnology and applied biochemistry (2021)
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