mFluor™ Violet 500 maleimide
mFluor™ Violet 500 Maleimide is an excellent building block that can be readily used for labeling biomolecules that have a free thiol (SH) group such as antibodies and thiol-modified oligos. mFluor™ Violet 500 dyes have fluorescence excitation and emission maxima of ~405 nm and ~500 nm respectively. These spectral characteristics make them an excellent replacement for Pacific Green™ (ThermoFisher) and BD Horizon™ V500 labeling dyes. mFluor™ Violet 500 Maleimide is reasonably stable and shows good reactivity and selectivity with free thiol group. mFluor™ Violet 500 Maleimide provides a convenient tool to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) for flow cytometric applications with the violet laser excitation. Under the same conditions, mFluor™ Violet 500 dye conjugates are significantly brighter than the corresponding bioconjugates of Pacific Green™ (ThermoFisher) and BD Horizon™ V500 with much stronger absorption, making the mFluor™ Violet 500 conjugates much more sensitive. AAT Bioquest's mFluor™ dyes are developed for multicolor flow cytometry-focused applications. These dyes have large Stokes Shifts, and can be well excited by the laser lines of flow cytometers (e.g., 405 nm, 488 nm and 633 nm).
Spectrum
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Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
mFluor™ Violet 500 SE | 410 | 501 | 250001 | 0.811 | 0.769 | 0.365 |
mFluor™ Violet 450 maleimide | 406 | 445 | 350001 | 0.811 | 0.338 | 0.078 |
mFluor™ Violet 530 maleimide | 393 | 543 | 200001 | - | - | - |
mFluor™ Violet 610 maleimide | 594 | 612 | 900001 | 0.31 | 0.532 | 0.66 |