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mFluor™ UV455-streptavidin conjugate
mFluor™ UV455 streptavidin conjugate consists of the tetrameric biotin-binding protein (streptavidin) covalently labeled with mFluor™ UV 455 fluorescent dyes. Its high affinity for biotinylated conjugates is leveraged across a wide range of biochemical applications for the specific detection of various proteins, protein motifs, nucleic acids, and other molecules. This concept is central to the workflow of several detection protocols, including western blots, flow cytometry, imaging and microscopy, and microplate assays, and is crucial in purification workflows aimed at targeted fractionation. The conjugation of mFluor™ UV455 with streptavidin is carefully optimized to maximize fluorescence intensity while preserving the inherent binding properties of streptavidin. Upon excitation by an ultraviolet laser, mFluor™ UV455 exhibits a bright and photostable emission of blue fluorescence at 461 nm. mFluor™ UV455 streptavidin conjugates are highly effective as second-step reagents for indirect immunofluorescent staining when used in conjunction with biotinylated primary antibodies.
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
| mFluor™ UV420-streptavidin conjugate | 353 | 421 | 750001 |
References
View all 12 references: Citation Explorer
Quantification of Small Molecule-Protein Interactions using FRET between Tryptophan and the Pacific Blue Fluorophore.
Authors: Lee, Molly M and Peterson, Blake R
Journal: ACS omega (2016): 1266-1276
Authors: Lee, Molly M and Peterson, Blake R
Journal: ACS omega (2016): 1266-1276
Fluorescence-based screening assays for the NAD⁺-dependent histone deacetylase smSirt2 from Schistosoma mansoni.
Authors: Schiedel, Matthias and Marek, Martin and Lancelot, Julien and Karaman, Berin and Almlöf, Ingrid and Schultz, Johan and Sippl, Wolfgang and Pierce, Raymond J and Romier, Christophe and Jung, Manfred
Journal: Journal of biomolecular screening (2015): 112-21
Authors: Schiedel, Matthias and Marek, Martin and Lancelot, Julien and Karaman, Berin and Almlöf, Ingrid and Schultz, Johan and Sippl, Wolfgang and Pierce, Raymond J and Romier, Christophe and Jung, Manfred
Journal: Journal of biomolecular screening (2015): 112-21
Discrimination between streptavidin and avidin with fluorescent affinity-based probes.
Authors: Sun, Qian and Tian, Haiyu and Qu, Haoran and Sun, Deheng and Chen, Zhuo and Duan, Liping and Zhang, Weibing and Qian, Junhong
Journal: The Analyst (2015): 4648-53
Authors: Sun, Qian and Tian, Haiyu and Qu, Haoran and Sun, Deheng and Chen, Zhuo and Duan, Liping and Zhang, Weibing and Qian, Junhong
Journal: The Analyst (2015): 4648-53
Rational design of biotinylated probes: fluorescent turn-on detection of (strept)avidin and bioimaging in cancer cells.
Authors: Sun, Qian and Qian, Junhong and Tian, Haiyu and Duan, Liping and Zhang, Weibing
Journal: Chemical communications (Cambridge, England) (2014): 8518-21
Authors: Sun, Qian and Qian, Junhong and Tian, Haiyu and Duan, Liping and Zhang, Weibing
Journal: Chemical communications (Cambridge, England) (2014): 8518-21
Discovery of coumarin derivatives as fluorescence acceptors for intrinsic fluorescence resonance energy transfer of proteins.
Authors: Kim, Ju Hwan and Sumranjit, Jitapa and Kang, Hyo Jin and Chung, Sang J
Journal: Molecular bioSystems (2014): 30-3
Authors: Kim, Ju Hwan and Sumranjit, Jitapa and Kang, Hyo Jin and Chung, Sang J
Journal: Molecular bioSystems (2014): 30-3
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