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mFluor™ UV420-streptavidin conjugate
mFluor™ UV420 streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with mFluor™ UV420 covalently attached. mFluor™ UV420 provides a unique color for fluorescence imaging and flow cytometric applications. It is used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. AAT Bioquest offers the largest collection of streptavidin conjugates. They are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. The affinity between streptavidin and biotin might be the strongest non-covalent interactions known in biological interactions. Streptavidin, a homotetrameric protein, exhibits an extraordinarily high affinity for biotin. Each streptavidin monomer can bind one biotin molecule, allowing a streptavidin protein to maximally bind four biotins. The streptavidin-biotin interaction is highly specific and remains robust under a wide range of conditions.
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| mFluor™ UV455-streptavidin conjugate | 357 | 461 | 200001 | 0.421 | 0.651 | 0.406 |
References
View all 11 references: Citation Explorer
Twelve Colors of Streptavidin-Fluorescent Proteins (SA-FPs): A Versatile Tool to Visualize Genetic Information in Single-Molecule DNA.
Authors: Jin, Yu and Bae, Jaeyoung and Kim, Tehee Yurie and Hwang, Hyeseung and Kim, Taesoo and Yu, Myungheon and Oh, Hyesoo and Hashiya, Kaori and Bando, Toshikazu and Sugiyama, Hiroshi and Jo, Kyubong
Journal: Analytical chemistry (2022): 16927-16935
Authors: Jin, Yu and Bae, Jaeyoung and Kim, Tehee Yurie and Hwang, Hyeseung and Kim, Taesoo and Yu, Myungheon and Oh, Hyesoo and Hashiya, Kaori and Bando, Toshikazu and Sugiyama, Hiroshi and Jo, Kyubong
Journal: Analytical chemistry (2022): 16927-16935
Surface functionalized thiol-ene waveguides for fluorescence biosensing in microfluidic devices.
Authors: Feidenhans'l, Nikolaj A and Lafleur, Josiane P and Jensen, Thomas G and Kutter, Jörg P
Journal: Electrophoresis (2014): 282-8
Authors: Feidenhans'l, Nikolaj A and Lafleur, Josiane P and Jensen, Thomas G and Kutter, Jörg P
Journal: Electrophoresis (2014): 282-8
Serum antiglycan antibody detection of nonmucinous ovarian cancers by using a printed glycan array.
Authors: Jacob, Francis and Goldstein, Darlene R and Bovin, Nicolai V and Pochechueva, Tatiana and Spengler, Marianne and Caduff, Rosemarie and Fink, Daniel and Vuskovic, Marko I and Huflejt, Margaret E and Heinzelmann-Schwarz, Viola
Journal: International journal of cancer (2012): 138-46
Authors: Jacob, Francis and Goldstein, Darlene R and Bovin, Nicolai V and Pochechueva, Tatiana and Spengler, Marianne and Caduff, Rosemarie and Fink, Daniel and Vuskovic, Marko I and Huflejt, Margaret E and Heinzelmann-Schwarz, Viola
Journal: International journal of cancer (2012): 138-46
Homogeneous competitive assay of ligand affinities based on quenching fluorescence of tyrosine/tryptophan residues in a protein via Főrster-resonance-energy-transfer.
Authors: Xie, Yanling and Yang, Xiaolan and Pu, Jun and Zhao, Yunsheng and Zhang, Ying and Xie, Guoming and Zheng, Jun and Yuan, Huidong and Liao, Fei
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2010): 869-76
Authors: Xie, Yanling and Yang, Xiaolan and Pu, Jun and Zhao, Yunsheng and Zhang, Ying and Xie, Guoming and Zheng, Jun and Yuan, Huidong and Liao, Fei
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2010): 869-76
[The biotin-thyroxin conjugate as a bifunctional ligand of binding proteins].
Authors: Novakovskiĭ, M E and Vashkevich, I I and Sviridov, O V
Journal: Bioorganicheskaia khimiia (2009): 178-91
Authors: Novakovskiĭ, M E and Vashkevich, I I and Sviridov, O V
Journal: Bioorganicheskaia khimiia (2009): 178-91
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