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mFluor™ UV375 Anti-human CD45 Antibody *HI30*

The HI30 monoclonal antibody recognizes human CD45, a 180 - 240 kD transmembrane glycoprotein typically found on the surface of B cells, neutrophils, hematopoietic cells and dendritic cells. CD45 is a component of vital cellular pathways, in particular, the regulation of receptor signaling pathway via JAK-STAT, B cell receptor signaling pathway and T cell receptor signaling pathway. Also, in certain organisms, it plays a role in the downregulation of cytokine-mediated signaling pathway, represses interleukin-2 biosynthetic process and is an enhancer of humoral immune response mediated by circulating immunoglobulin. CD45 has been thought to be involved with critical biological processes such as dephosphorylation, especially protein dephosphorylation, and is associated with a variety of biologically interesting macromolecules/ligands, for instance, p56lck. CD45 is a very popular antibody target, with over 50000 publications in the last decade. CD45 is often used in flow cytometry applications as a phenotypic marker for differentiation of cell types, particularly in the study of neuroscience, immunology and neuroscience cell markers. This antibody was purified through affinity chromatography and conjugated to mFluor™ UV375 (ex/em = 351/387 nm). It is compatible with the 355 nm laser and 387/11 nm bandpass filter (for example, as in the Bio-Rad ZE5 Cell Analyzer).

Spectrum

Conjugation

We provide custom conjugation services for this antibody (eg. labeling of cd45 antibody with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.
Page updated on October 30, 2024

Ordering information

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Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Antibody properties

Other namesLeukocyte Common Antigen (LCA); T200; PTPRC
CloneHI30
Hostmouse
Isotypemouse IgG1
Reactivityhuman
ApplicationFlow Cytometry (FACS); Fluorescence Imaging

Spectral properties

Absorbance (nm)

342

Correction Factor (260 nm)

0.099

Correction Factor (280 nm)

0.138

Extinction coefficient (cm -1 M -1)

300001

Excitation (nm)

351

Emission (nm)

387

Quantum yield

0.941

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
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