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mFluor™ UV 375 goat anti-rabbit IgG (H+L)

mFluor™ UV375 goat anti-rabbit conjugates are secondary antibodies designed for optimal performance in immunoassay applications, including flow cytometry, immunofluorescence, and confocal microscopy. These conjugates consist of goat-derived polyclonal antibodies with high affinity and specificity towards rabbit IgG, conjugated to the bright and stable mFluor™ UV375 fluorochrome. This conjugation is optimized to ensure minimal non-specific binding and enhanced signal clarity, with rigorous purification steps to remove unconjugated components. Provided in a ready-to-use format with a recommended dilution range, the conjugate undergoes stringent quality control tests for performance and specificity. Its compatibility with a wide range of rabbit primary antibodies and the exceptional contrast provided by mFluor™ UV375 fluorescence makes it a reliable tool for detecting diverse target antigens in multicolor staining protocols. mFluor™ UV375 is optimally excited by the UV laser and emits maximally at 387 nm. These affinity-purified goat anti-rabbit secondary antibodies are valuable for their versatility and sensitivity, enabling efficient detection, sorting, or purification of specific targets through effective signal amplification in research applications.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
mFluor™ UV 375 goat anti-mouse IgG (H+L)3513873000010.9410.0990.138
mFluor™ UV 375 goat anti-mouse IgG (H+L) *Cross-Absorbed*3513873000010.9410.0990.138

References

View all 50 references: Citation Explorer
Development of a Competitive Chemiluminescent Assay for Quantitative Determination of TP53 Fusion Protein Using Reagent Strips.
Authors: Wang, Linyu and He, Zhifang and Li, Jianye
Journal: Applied biochemistry and biotechnology (2024)
Functional Association between Storage Protein Mobilization and Redox Signaling in Narrow-Leafed Lupin (Lupinus angustifolius L.) Seed Germination and Seedling Development.
Authors: Escudero-Feliu, Julia and Lima-Cabello, Elena and Rodríguez de Haro, Esther and Morales-Santana, Sonia and Jimenez-Lopez, Jose C
Journal: Genes (2023)
Establishment of an indirect ELISA for Mycobacterium tuberculosis MTB39A protein antibody.
Authors: Wang, Pu and Cai, Yurong and Zhang, Gang and Jiang, Lingling and Li, Yong
Journal: Applied microbiology and biotechnology (2023): 6339-6349
Development of an immunochromatographic strip test for antigen detection of elephant endotheliotropic herpesvirus in Asian elephants (Elephas maximus).
Authors: Guntawang, Thunyamas and Sittisak, Tidaratt and Srivorakul, Saralee and Photichai, Kornravee and Aiumurai, Pisinee and Thitaram, Chatchote and Sthitmatee, Nattawooti and Hsu, Wei-Li and Sookrung, Nitat and Pringproa, Kidsadagon
Journal: Journal of virological methods (2023): 114627
Silica-coated gold nanorods biofunctionalization for localized surface plasmon resonance (LSPR) biosensing.
Authors: Pellas, Vincent and Sallem, Fadoua and Blanchard, Juliette and Miche, Antoine and Concheso, Sara Martinez and Méthivier, Christophe and Salmain, Michèle and Boujday, Souhir
Journal: Talanta (2023): 124245
Page updated on December 17, 2024

Ordering information

Price
Unit size
200 ug
1 mg
Catalog Number
4920049201
Quantity
Add to cart

Additional ordering information

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Physical properties

Molecular weight

~150 kDa

Solvent

Water

Spectral properties

Absorbance (nm)

342

Correction Factor (260 nm)

0.099

Correction Factor (280 nm)

0.138

Extinction coefficient (cm -1 M -1)

300001

Excitation (nm)

351

Emission (nm)

387

Quantum yield

0.941

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501