mFluor™ Red 780 Biotin Conjugate
mFluor™ Red 780 Biotin Conjugate contains mFluor™ Red 780 fluorophore that is well excited by the common 350 nm laser. It is a unique color for spectral flow cytometry, and demonstrates excellent avidin binding and strong near intrared (NIR) fluorescence. It has avidin-binding properties similar to native biotin in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers. mFluor™ Red 780 Biotin Conjugate can be used for detecting avidin, streptavidin or neutravidin conjugates. It is a water-soluble reagent and has pH independent fluorescence from pH 4 to pH 10. The flexible spacer between biotin moiety and fluorescent tag minimizes the steric hindrance involved in binding to avidin, streptavidin or neutravidin.
Spectrum
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Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
mFluor™ Red 780-streptavidin conjugate | 629 | 767 | 900001 | 0.101 | 0.116 |
References
View all 20 references: Citation Explorer
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