mFluor™ Blue 630 SE

mFluor™ Blue 630 dye can be well excited with blue laser at 488 nm. It has a huge Stokes shift with emission ~630 nm. mFluor™ Blue 630 dyes are water-soluble, and the protein conjugates prepared with mFluor™ Blue 630 dyes are well excited at 488 nm to give red fluorescence. mFluor™ Blue 630 dye and conjugates are excellent blue laser reagents for flow cytometry detections. Compared to RPE, mFluor™ Blue 630 dyes are much more photostable, making them readily available for fluorescence imaging applications while it is very difficult to use RPE conjugates for fluorescence imaging applications due to the rapid photobleaching of RPE conjugates. It is also a unique fluorochrome for spectral flow cytometry since there are very few existing dyes that have this spectral profile.

Fluorescent dye NHS esters (or succinimidyl esters) are the most popular tool for conjugating dyes to a peptide, protein, antibody, amino-modified oligonucleotide, or nucleic acid. NHS esters react readily with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
1164	1 mg	Price

References

View all 19 references: Citation Explorer

A Comprehensive Workflow for Applying Single-Cell Clustering and Pseudotime Analysis to Flow Cytometry Data.

Authors:

Melsen, Janine E and van Ostaijen-Ten Dam, Monique M and Lankester, Arjan C and Schilham, Marco W and van den Akker, Erik B

Journal:

Journal of immunology (Baltimore, Md. : 1950) (2020)

High-Dimensional Data Analysis Algorithms Yield Comparable Results for Mass Cytometry and Spectral Flow Cytometry Data.

Authors:

Ferrer-Font, Laura and Mayer, Johannes U and Old, Samuel and Hermans, Ian F and Irish, Jonathan and Price, Kylie M

Journal:

Cytometry. Part A : the journal of the International Society for Analytical Cytology (2020)

HTLV infected individuals have increased B-cell activation and proinflammatory regulatory T-cells.

Authors:

Kjerulff, Bertram and Petersen, Mikkel Steen and Rodrigues, Candida Medina and da Silva Té, David and Christiansen, Mette and Erikstrup, Christian and Hønge, Bo Langhoff

Journal:

Immunobiology (2020): 151878

Multispectral Flow Cytometry: Unaddressed Issues and Recommendations for Improvement.

Authors:

Parks, David R

Journal:

Cytometry. Part A : the journal of the International Society for Analytical Cytology (2020)

High-dimensional analysis of intestinal immune cells during helminth infection.

Authors:

Ferrer-Font, Laura and Mehta, Palak and Harmos, Phoebe and Schmidt, Alfonso J and Chappell, Sally and Price, Kylie M and Hermans, Ian F and Ronchese, Franca and le Gros, Graham and Mayer, Johannes U

Journal:

eLife (2020)

Physical properties

Molecular weight	709.86
Solvent	DMSO

Spectral properties

Absorbance (nm)	472
Correction factor (260 nm)	0.197
Correction factor (280 nm)	0.275
Extinction coefficient (cm ^-1 M ^-1)	49000 ¹
Excitation (nm)	470
Emission (nm)	632

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on October 15, 2025