Methylene Blue Maleimide

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Physical properties

Molecular weight	577.58
Solvent	DMSO

Spectral properties

Absorbance (nm)

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Alternative formats

Overview SDS Protocol

Molecular weight

577.58

Absorbance (nm)

662

Methylene Blue (MB) is widely used as a redox indicator in nucleic acid research. Methylene Blue maleimide is an excellent building block for preparing MB-labeled nucleic acid probes from thiol-modified oligonucleotides. MB is also a well-known photosensitizer used in photodynamic therapy (PDT) and investigated for the topical treatment of various skin infections via PDT modality. It is a common biological stain, commonly used in medical laboratories to stain cells and tissues for microscopic examination. It can help visualize cellular structures and identify abnormalities. In addition, MB has been studied for its potential role in treating neurodegenerative diseases, such as Alzheimer's and Parkinson's disease. Some research suggests it might have a protective effect on neurons and mitochondrial function.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Methylene Blue Maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	173.136 µL	865.681 µL	1.731 mL	8.657 mL	17.314 mL
5 mM	34.627 µL	173.136 µL	346.272 µL	1.731 mL	3.463 mL
10 mM	17.314 µL	86.568 µL	173.136 µL	865.681 µL	1.731 mL

Molarity calculator

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Spectrum

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Spectral properties

Absorbance (nm)

662

Images

Figure 1. Methylene Blue (MB) is widely used as a redox indicator in nucleic acid research. Methylene Blue maleimide is an excellent building block for preparing MB-labeled nucleic acid probes from thiol-modified oligonucleotides.

References

View all 18 references: Citation Explorer

Solution-Phase Electrochemical Aptamer-Based Sensors.
Authors: Yuan, Yuchan and Bali, Ahilya and White, Ryan J and Heikenfeld, Jason
Journal: IEEE transactions on bio-medical engineering (2023): 824-830

Design and 3D printing of an electrochemical sensor for Listeria monocytogenes detection based on loop mediated isothermal amplification.
Authors: Rivas-Macho, Ane and Eletxigerra, Unai and Diez-Ahedo, Ruth and Merino, Santos and Sanjuan, Antton and Bou-Ali, M Mounir and Ruiz-Rubio, Leire and Del Campo, Javier and Vilas-Vilela, José Luis and Goñi-de-Cerio, Felipe and Olabarria, Garbiñe
Journal: Heliyon (2023): e12637

Nitrogen-Doped 4H Silicon Carbide Single-Crystal Electrode for Selective Electrochemical Sensing of Dopamine.
Authors: Fathi, Fatemeh and Sueoka, Brandon and Zhao, Feng and Zeng, Xiangqun
Journal: Analytical chemistry (2023): 4855-4862

Fabrication of Au/Fe3O4/RGO based aptasensor for measurement of miRNA-128, a biomarker for acute lymphoblastic leukemia (ALL).
Authors: Dinani, Homayoon Soleimani and Pourmadadi, Mehrab and Yazdian, Fatemeh and Rashedi, Hamid and Ebrahimi, Seyed Ali Seyed and Shayeh, Javad Shabani and Ghorbani, Mehdi
Journal: Engineering in life sciences (2022): 519-534

In vivo application of an implantable tri-anchored methylene blue-based electrochemical pH sensor.
Authors: González-Fernández, Eva and Staderini, Matteo and Marland, Jamie R K and Gray, Mark E and Uçar, Ahmet and Dunare, Camelia and Blair, Ewen O and Sullivan, Paul and Tsiamis, Andreas and Greenhalgh, Stephen N and Gregson, Rachael and Clutton, Richard Eddie and Smith, Stewart and Terry, Jonathan G and Argyle, David J and Walton, Anthony J and Mount, Andrew R and Bradley, Mark and Murray, Alan F
Journal: Biosensors & bioelectronics (2022): 113728

Improved Thermal Stability and Homogeneity of Low Probe Density DNA SAMs Using Potential-Assisted Thiol-Exchange Assembly Methods.
Authors: Ma, Tianxiao and Bizzotto, Dan
Journal: Analytical chemistry (2021): 15973-15981

A PCR-free screen-printed magnetic electrode for the detection of circular RNA from hepatocellular cancer based on a back-splice junction.
Authors: Zhang, Bin and Liang, Yitao and Bo, Liang and Chen, Mingyu and Huang, Bobo and Cao, Qingpeng and Wei, Jinwei and Li, Tianyu and Cai, Xiujun and Ye, Xuesong
Journal: RSC advances (2021): 17769-17774

Reversible filtration redox of methylene blue in dimethylsulfoxide by manganese oxide loaded carbonaceous nanofibrous membrane through Fenton-like oxidation.
Authors: Wang, Yang and Wang, Jianqiang and Li, Guiliang and Geng, Xiaolan and Hu, Tuoping and Liu, Fu
Journal: Journal of colloid and interface science (2021): 436-445

Hybrid Nanocomposite Platform, Based on Carbon Nanotubes and Poly(Methylene Blue) Redox Polymer Synthesized in Ethaline Deep Eutectic Solvent for Electrochemical Determination of 5-Aminosalicylic Acid.
Authors: Hosu, Oana and Barsan, Madalina M and Săndulescu, Robert and Cristea, Cecilia and Brett, Christopher M A
Journal: Sensors (Basel, Switzerland) (2021)

Calibration-Free Measurement of Phenylalanine Levels in the Blood Using an Electrochemical Aptamer-Based Sensor Suitable for Point-of-Care Applications.
Authors: Idili, Andrea and Parolo, Claudio and Ortega, Gabriel and Plaxco, Kevin W
Journal: ACS sensors (2019): 3227-3233